CAJ | 학술논문

聚合酶链式反应是常规分子生物学实验的重要内容之一。作者在多年从事分子生物学实验教学的基础上，根据聚合酶链式反应实验特点，在实验操作的间隔时段引入相关问题的探讨。整个教学过程由学生查阅资料进行问题和实验准备、教师对实验原理与操作注意事项的简单介绍、实验操作、问题讨论、实验结果观察与分析等组成。实施效果表明，作者采用的教学组织方案能够更加有效地利用实验过程中的间歇时间，通过对预先设定的问题和学生自主提问进行探讨，实现良好的师生互动，加深了学生对聚合酶链式反应实验原理及相关理论的理解与掌握，增强学生利用所学理论知识分析实际问题的能力。对于激发学生的学习积极性具有较好的促进作用。
취합매련식반응시상규분자생물학실험적중요내용지일。작자재다년종사분자생물학실험교학적기출상，근거취합매련식반응실험특점，재실험조작적간격시단인입상관문제적탐토。정개교학과정유학생사열자료진행문제화실험준비、교사대실험원리여조작주의사항적간단개소、실험조작、문제토론、실험결과관찰여분석등조성。실시효과표명，작자채용적교학조직방안능구경가유효지이용실험과정중적간헐시간，통과대예선설정적문제화학생자주제문진행탐토，실현량호적사생호동，가심료학생대취합매련식반응실험원리급상관이론적리해여장악，증강학생이용소학이론지식분석실제문제적능력。대우격발학생적학습적겁성구유교호적촉진작용。
Polymerase chain reaction is a powerful tool for in vitro amplication of specic nucleic acid se-quences and has been assigned one of the important content of the conventional molecular biology ex-periments as its wide usage in life science related subjects and its impact on the scientic community in-volving in basic research and practical analysis .The author provide a practicable teaching scheme focu-sing on the quality-oriented education within which problem discussion was properly introduced into the course during running polymerase chain reaction and/or the quench time of electrophoresis .The w hole process of this experiment includes problem preparation ,teacher’s explanation ,students’ op-eration ,problem discussion ,observation and analysis of the experiment result . Teaching practice proved that this scheme can effectively use the interval time ,through problem discussion ,to stimu-late the interaction and communication between teachers and students ,help students of to understand the principle and related theoretical aspects of polymerase chain reaction ,enhance the students’ ability of using theoretical knowledge to analysis the actual problem .Learning interest can be also promoted .