CAJ | 학술논문

目的：研究大鼠心肌缺血、缺血再灌注、无创性缺血预处理以及氯化锂干预后心肌细胞β结合素的阳性表达率及细胞凋亡指数的变化，以探讨β结合素与心肌缺血、缺血再灌注的关系以及可能机制。方法将入选的60只大鼠随机分为假手术对照组（C ）、缺血组（I ）、缺血再灌注组（IR ）、氯化锂药物预处理组（PPC ）。以TUNEL方法评估各组大鼠心肌细胞凋亡情况，以免疫组化方法测定各组大鼠心肌细胞中β结合素的表达，并计算各组大鼠的心肌细胞凋亡指数及β结合素。结果与C组比较，I、IR、PPC四组凋亡指数均升高（ P＜0.05），各组心肌细胞凋亡指数比较的结果为：IR组＞I组＞PPC组＞C组（各组两两比较均 P＜0.05）；各组心肌细胞β_cat阳性表达率比较的结果为：PPC组＞C组＞I组＞IR组。β_cat阳性表达率与凋亡指数呈负相关，R＝-0.90。结论β_cat阳性表达率与凋亡指数呈负相关，提示β_cat可以减少心肌缺血、缺血再灌注引起的细胞凋亡。
목적：연구대서심기결혈、결혈재관주、무창성결혈예처리이급록화리간예후심기세포β결합소적양성표체솔급세포조망지수적변화，이탐토β결합소여심기결혈、결혈재관주적관계이급가능궤제。방법장입선적60지대서수궤분위가수술대조조（C ）、결혈조（I ）、결혈재관주조（IR ）、록화리약물예처리조（PPC ）。이TUNEL방법평고각조대서심기세포조망정황，이면역조화방법측정각조대서심기세포중β결합소적표체，병계산각조대서적심기세포조망지수급β결합소。결과여C조비교，I、IR、PPC사조조망지수균승고（ P＜0.05），각조심기세포조망지수비교적결과위：IR조＞I조＞PPC조＞C조（각조량량비교균 P＜0.05）；각조심기세포β_cat양성표체솔비교적결과위：PPC조＞C조＞I조＞IR조。β_cat양성표체솔여조망지수정부상관，R＝-0.90。결론β_cat양성표체솔여조망지수정부상관，제시β_cat가이감소심기결혈、결혈재관주인기적세포조망。
Objective To explore the relationship and possible related mechanisms between β_cat ,myocardial ischemia and ischemia reperfusion ,the changes of apoptotic index and positive expression index of β_cat in rats with myocardial ischemia ,ischemia_reperfu-sion ,ischemic preconditioning and lithium chloride intervention were observed respectively. Methods 60 SD (standard ) rats were randomly divided into 5 groups as follows:Control group (C) ,Ischemic group (I) ,Ischemia_reperfusion group (IR) ,Ischemic pre-condition group (IPC) ,Pharmacological preconditioning group (PPC).The level of myocardial apoptosis was detected by TUNEL , the expression of β_cat was detected by Immunohistochemistry ,the apoptotic index andβ_cat of each group were measured. Results Compared with group C ,in group I ,IR ,IPC and PPC ,the apoptotic indexes were all increased ( P<0.05);the comparison of apoptotic index in each group was as follows :IR group>I group>IPC group>PPC group>C group ,there were significant differ-ences among groups ( P<0.05 );comparison of the positive expression indexes of β_cat:PPC group>IPC group>C group>I group>IR group.The positive expression index of β_cat was negatively correlated with AI ,R= -0.90.Conclusions 1.The positive expres-sion index ofβ_cat was negatively correlated with AI.The results indicated that β_cat could reduce apoptosis in the situation of my-ocardial ischemia and ischemia reperfusion.2.The IPC group has strongerβ_cat expression and lower AI than IR and I group ,which indicated the anti_apoptosis effect may have relationship with the stranger expression of β_cat.