CAJ | 학술논문

对14份土样和7株实验室保藏菌株经初筛、摇瓶复筛,得到1株油脂产量较高的酵母菌,其油脂含量为7.34%。对影响该菌体产油脂的条件进行了单因素和正交实验,得出摇瓶发酵培养的最佳产油脂条件：发酵周期为4 d,酵母膏和硫酸铵混合物为氮源,接种量为12%。在该条件下测得的菌体生物量为12.211 g/L,油脂产量为1.524 g/L,油脂含量为12.48%。
대14빈토양화7주실험실보장균주경초사、요병복사,득도1주유지산량교고적효모균,기유지함량위7.34%。대영향해균체산유지적조건진행료단인소화정교실험,득출요병발효배양적최가산유지조건：발효주기위4 d,효모고화류산안혼합물위담원,접충량위12%。재해조건하측득적균체생물량위12.211 g/L,유지산량위1.524 g/L,유지함량위12.48%。
After preliminary screening and shake flask screening of the 14 soil samples and 7 strains preserved in the laboratory,the authors of this paper obtained a rich oil-yielding yeast whose fat content is 7.34%.The single factor and orthogonal experiments on the microbial lipid production conditions indicate the optimum conditions of producing oil in shaking flask culture. The fermentation period is 4 days,and when the source of yeast extract and the mixture of am-monium sulfate is nitrogen,the inoculation is 1 2%.Under these conditions the measured biomass is 12.211 g/L,oil output is1.524 g/L,and oil content is 12.48%.