CAJ | 학술논문

目的：通过观察半夏厚朴汤对慢性应激抑郁模型大鼠行为学和氧化应激的影响，研究半夏厚朴汤抗抑郁的机制。方法：采用强迫游泳实验、开野实验、糖水偏好实验，研究半夏厚朴汤对慢性应激抑郁模型大鼠的抗抑郁作用，并通过UPLC-T-QMS系统检测其大鼠脑内单胺类神经递质的含量，部分动物取其海马制备海马匀浆，Western blotting检测超氧化物歧化酶（SOD）、丙二醛（MDA）。结果：与空白组相比，模型组大鼠糖水消耗量显著下降，强迫游泳不动时间显著延长，交叉次数、直立次数显著减少；海马内去甲肾上腺素、5-羟色胺含量显著降低。与模型组相比，半夏厚朴汤组糖水消耗量显著升高，强迫游泳不动时间显著缩短。半夏厚朴汤组显著增加大鼠脑内去甲肾上腺素和5-羟色胺含量。Western blotting检测结果显示，与模型组比较，半夏厚朴汤组显著升高SOD水平，显著降低MDA水平。结论：半夏厚朴汤能明显改善慢性应激所致的大鼠抑郁行为，其机制可能与上调海马内去甲肾上腺素、5-羟色胺水平，增强机体抗氧化应激能力有关。
목적：통과관찰반하후박탕대만성응격억욱모형대서행위학화양화응격적영향，연구반하후박탕항억욱적궤제。방법：채용강박유영실험、개야실험、당수편호실험，연구반하후박탕대만성응격억욱모형대서적항억욱작용，병통과UPLC-T-QMS계통검측기대서뇌내단알류신경체질적함량，부분동물취기해마제비해마균장，Western blotting검측초양화물기화매（SOD）、병이철（MDA）。결과：여공백조상비，모형조대서당수소모량현저하강，강박유영불동시간현저연장，교차차수、직립차수현저감소；해마내거갑신상선소、5-간색알함량현저강저。여모형조상비，반하후박탕조당수소모량현저승고，강박유영불동시간현저축단。반하후박탕조현저증가대서뇌내거갑신상선소화5-간색알함량。Western blotting검측결과현시，여모형조비교，반하후박탕조현저승고SOD수평，현저강저MDA수평。결론：반하후박탕능명현개선만성응격소치적대서억욱행위，기궤제가능여상조해마내거갑신상선소、5-간색알수평，증강궤체항양화응격능력유관。
Objectiv e: To study the anti-depression mechanism of Banxia Houpu Decoction. Methods:Rat models were established by chronic unpredictable mild stress (CUMS), sucrose consumption, open-field and forced swimming tests were used to observe the behavior of the model rats. UPLC-T-QMS was used to analyze the levels of monoamine neurotransmitters. Western blotting detected the levels of superoxide dis-mutase(SOD) and malondialdehyde(MDA) in brain. Results: Compared with the normal group, the frequency of crossing and standing and the consumption of sucrose water in the depressive disorder rats decreased significantly, the duration of immobility in forced swimming test increased significantly, the contents of 5-HT and NA reduced. Compared with the model group, the frequency of crossing and standing and the consumption of sucrose water in the Banxia Houpu Decoction group rats increased significantly, the dura-tion of immobility in forced swimming test decreased significantly. Western blotting analysis showed that, compared with the model group, Banxia Houpu Decoction could significantly elevate the levels of SOD, and significantly reduce the levels of MDA in hippocampus. Conclusion: Banxia Houpu Decoction improved the depression syndromes of rats induced by CUMS, of which the mechanism involved the upregulation of 5-HT and NE levels and enhancement of anti-oxidative stress ability.