CAJ | 학술논문

大黄鱼养殖业中存在因过量用药而造成环境污染严重及大黄鱼发病率和死亡率高等问题，以淀粉、壳聚糖和明胶三元复合物作为载体，以渔业中应用较多的广谱抗生素氟苯尼考为药物模型，制备了一种可生物降解并可定量给药的抗生素药丸。采用扫描电子显微镜、高效液相色谱法等手段对该药丸的物理特性、吸水性能、抑菌性能及药物残留量进行了检测。结果表明：所制备的载体和药丸具有一致的内部微观结构，多孔结构分布均匀，孔与孔之间具有良好的连通性，有利于药物的流通和释放。体外抑菌试验结果显示，其抑菌能力与氟苯尼考的含量成正相关。经口服药丸后，氟苯尼考在大黄鱼血液、肝脏和肌肉中的药代动力学符合一级吸收二室开放模型的特征，并可在血液、肝脏和肌肉中缓慢消解。
대황어양식업중존재인과량용약이조성배경오염엄중급대황어발병솔화사망솔고등문제，이정분、각취당화명효삼원복합물작위재체，이어업중응용교다적엄보항생소불분니고위약물모형，제비료일충가생물강해병가정량급약적항생소약환。채용소묘전자현미경、고효액상색보법등수단대해약환적물리특성、흡수성능、억균성능급약물잔류량진행료검측。결과표명：소제비적재체화약환구유일치적내부미관결구，다공결구분포균균，공여공지간구유량호적련통성，유리우약물적류통화석방。체외억균시험결과현시，기억균능력여불분니고적함량성정상관。경구복약환후，불분니고재대황어혈액、간장화기육중적약대동역학부합일급흡수이실개방모형적특정，병가재혈액、간장화기육중완만소해。
Problems such as drug abusiveness, poor efficacy, nonquantitatively medicine and improper administration raised high morbidity and mortality in Pseudosciaena crocea fishery. A biodegradable antibiotic pill was manufactured, in which starch, chitosan and gelatin were used as carriers and florfenicol, a broad spectrum antibiotic in fishery, was used as the model drug. The carrier's morphology, water uptake, bacteriostasis capability and drug-residue of the pill were analyzed using scanning electron microscopy ( SEM) , high performance liquid chromatography ( HPLC) , in vitro and in vivo test. The microscopic structures of both carrier and pill were quite uniform in pore size and pore distribution with good interconnectivity between pores, which is favorable for the drug's circulation and release. The in vitro bacteriostasis test showed that the pills were effective against Vibrio alginolyticus while the carrier materials were little antimicrobial active. The in vivo toxicity, pharmacokinetics and drug residues as a function of time were tested with Pseudosciaena crocea as the test fish. The blood, liver and muscle of tested fishes were obtained and evaluated. The results showed that the carrier materials were low toxic for Pseudosciaena crocea. 5 -7 days after oral administration, trace level residue of florfenicol was tested in blood, liver and muscle tissue of Pseudosciaena crocea. The pharmacokinetics was in line with a two-compartment open model with first-order absorption factor.