CAJ | 학술논문

为评价新型季酮酸衍生物类杀虫剂螺虫乙酯（ spirotetramat）对水生生物的毒性，以雌性斑马鱼为受试对象，研究了螺虫乙酯对其的急性毒性及氧化应激效应。急性毒性试验测得螺虫乙酯对雌性斑马鱼的96 h-LC50值为7?21 mg/L，属中等毒性。经不同浓度螺虫乙酯（0、36、360和1800μg/L）处理28 d后，通过检测铜/锌超氧化物歧化酶（ Cu/Zn-SOD）、锰超氧化物歧化酶（Mn-SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）等抗氧化酶的活性，Cat、Gpx、Mn-Sod、Cu/Zn-Sod等基因的相对表达量，以及丙二醛（ MDA ）含量等指标的变化水平，研究了螺虫乙酯对斑马鱼肌肉组织的氧化应激效应。结果表明，螺虫乙酯可引起斑马鱼肌肉组织中CAT、GSH-Px活性和MDA含量，以及Cat、Mn-Sod、Gpx的相对表达量显著升高，但未发现螺虫乙酯对Cu/Zn-SOD活性及Cu/Zn-Sod的相对表达量有明显影响。研究表明，螺虫乙酯能够诱导雌性斑马鱼肌肉组织的氧化应激效应，并可能引起机体的氧化损伤。
위평개신형계동산연생물류살충제라충을지（ spirotetramat）대수생생물적독성，이자성반마어위수시대상，연구료라충을지대기적급성독성급양화응격효응。급성독성시험측득라충을지대자성반마어적96 h-LC50치위7?21 mg/L，속중등독성。경불동농도라충을지（0、36、360화1800μg/L）처리28 d후，통과검측동/자초양화물기화매（ Cu/Zn-SOD）、맹초양화물기화매（Mn-SOD）、과양화경매（CAT）화곡광감태과양화물매（GSH-Px）등항양화매적활성，Cat、Gpx、Mn-Sod、Cu/Zn-Sod등기인적상대표체량，이급병이철（ MDA ）함량등지표적변화수평，연구료라충을지대반마어기육조직적양화응격효응。결과표명，라충을지가인기반마어기육조직중CAT、GSH-Px활성화MDA함량，이급Cat、Mn-Sod、Gpx적상대표체량현저승고，단미발현라충을지대Cu/Zn-SOD활성급Cu/Zn-Sod적상대표체량유명현영향。연구표명，라충을지능구유도자성반마어기육조직적양화응격효응，병가능인기궤체적양화손상。
In order to evaluate the aquatic toxicity of spirotetramat, a new tetramic acid derivative, the acute toxicity and oxidative stress of spirotetramat in female zebrafish( Danio rerio) was studied. The result of acute toxicity test showed that the 96 h-LC50 value of spirotetramat was 7?21 mg/L, suggesting spirotetramat has moderate toxicity to female zebrafish. Oxidative stress was processed by assaying antioxidant enzyme activities(CAT, Cu/Zn-SOD, Mn-SOD and GSH-Px), relative mRNA expression levels of genes( Cat, Cu/Zn-Sod, Mn-Sod, Gpx) and MDA content in zebrafish muscle after exposing to various mass concentrations(0, 36, 360 and 1 800 μg/L) of spirotetramat for 28 d. The results indicated that spirotetramat could induce significant increase of the CAT and GSH-Px activities, MDA content, and mRNA expression levels of Cat, Mn-Sod and Gpx in zebrafish muscle;while the Cu/Zn-Sod mRNA expression level and Cu/Zn-SOD activity was not changed significantly. The results suggested that oxidative stress was induced in zebrafish muscle by spirotetramat, which may cause oxidative damage in the fish.