CAJ | 학술논문

目的：研究鱼藤酮对大鼠脑内5-羟色胺能神经元的毒性作用及其机制。方法健康成年雄性Wistar大鼠背部皮下注射鱼藤酮制备帕金森病动物模型。采用免疫组化、蛋白印迹及分光吸光度法检测大鼠中脑中缝背核5-羟色胺能神经元的损伤及中缝背核氧化应激参数（丙二醛和还原型谷胱甘肽）的改变。结果和对照组相比，鱼藤酮组大鼠脑内中缝背核5-羟色胺（5-HT）免疫反应阳性神经元数明显少于对照组（P＜0．01），Western blot结果显示中缝背核5-HT表达在鱼藤酮组明显降低（ P＜0．01）；鱼藤酮组大鼠中缝背核区域丙二醛含量明显增高（ P＜0．01）、还原型谷胱甘肽（ GSH）含量明显减少（ P＜0．01）。结论鱼藤酮在损伤大鼠脑内多巴胺能神经元的同时对5-HT能神经元也具有明显的毒性作用，氧化应激可能是导致5-HT神经元损伤的主要原因。
목적：연구어등동대대서뇌내5-간색알능신경원적독성작용급기궤제。방법건강성년웅성Wistar대서배부피하주사어등동제비파금삼병동물모형。채용면역조화、단백인적급분광흡광도법검측대서중뇌중봉배핵5-간색알능신경원적손상급중봉배핵양화응격삼수（병이철화환원형곡광감태）적개변。결과화대조조상비，어등동조대서뇌내중봉배핵5-간색알（5-HT）면역반응양성신경원수명현소우대조조（P＜0．01），Western blot결과현시중봉배핵5-HT표체재어등동조명현강저（ P＜0．01）；어등동조대서중봉배핵구역병이철함량명현증고（ P＜0．01）、환원형곡광감태（ GSH）함량명현감소（ P＜0．01）。결론어등동재손상대서뇌내다파알능신경원적동시대5-HT능신경원야구유명현적독성작용，양화응격가능시도치5-HT신경원손상적주요원인。
Objective To study the toxic mechanism of rotenone on serotonergic neurons of brain in rats .Methods Healthy adult male Wistar rats were injected rotenone subcutaneous to prepare for Parkinson's disease animal model.The damage of serotonergic neurons of the dorsal raphe nucleus in rats brain was detected using immunocytochemistry, Western blot and spectrophotometry techniques.Results The numbers of 5-HT immune response positive neuron significantly reduced in the dorsal raphe nucleus of rotenone group as compare with control group (P<0.01).The results of Western blot showed that the expression of 5-HT in the dorsal raphe nucleus was significantly decreased in rotenone group ras (P<0.01).Compared with control group, oxidative damage significantly increased in the dorsal raphe nucleus of rotenone group rats.Conclusion Rotenone bears obvious toxicity to serotonergic neurons, and oxidative stress might be the may pathogenesis of rotenone on serotonergic neurons in rats.