CAJ | 학술논문

建立婴幼儿营养米粉中黄曲霉毒素B1的高效液相色谱荧光检测器测定方法。样品以甲醇-水(体积比70∶30)溶液匀质提取，过黄曲霉毒素B1免疫层析亲和柱净化，经CNW Athena C18色谱柱分离和光化学柱后衍生反应器衍生后，用带有荧光检测器的高效液相色谱仪测定。采用峰面积外标法定量黄曲霉毒素B1含量。黄曲霉毒素B1在0～10μg/L的浓度范围内线性关系良好，相关系数为0.9998，检出限为0.25μg/kg。在3个添加水平下加标回收率为97.7%～106.9%，测定结果的相对标准偏差为1.7%(n=6)。该方法的灵敏度、准确度、精密度均符合黄曲霉毒素B1的检测技术要求，适用于婴幼儿营养米粉中黄曲霉毒素B1的日常检测。
건립영유인영양미분중황곡매독소B1적고효액상색보형광검측기측정방법。양품이갑순-수(체적비70∶30)용액균질제취，과황곡매독소B1면역층석친화주정화，경CNW Athena C18색보주분리화광화학주후연생반응기연생후，용대유형광검측기적고효액상색보의측정。채용봉면적외표법정량황곡매독소B1함량。황곡매독소B1재0～10μg/L적농도범위내선성관계량호，상관계수위0.9998，검출한위0.25μg/kg。재3개첨가수평하가표회수솔위97.7%～106.9%，측정결과적상대표준편차위1.7%(n=6)。해방법적령민도、준학도、정밀도균부합황곡매독소B1적검측기술요구，괄용우영유인영양미분중황곡매독소B1적일상검측。
A high performance liquid chromatography(HPLC) coupled with post-column photochemical derivatization method was developed for the determination of aflatoxin B1 in rice cereal for infants and young children. The sample was extracted with methanol-water (volume ratio was 70∶30) then purified by immunoaffinity column for the purification aflatoxin B1. The high performance liquid chromatography (HPLC) separation was performed on a CNW Athena C18 column with methanol and water. The aflatoxin B1 was determined by fluorescence detection (FLD) after photochemical derivatization,and quantified by the peak area external standard method. The calibration curve showed good linearity in 0-10 μg/L range with correlation coefficient of 0.999 8. The limit of detection (LOD) was 0.25μg/kg for aflatoxin B1 in rice cereal for infants and young children. The recovery of aflatoxin B1 spiked at there levels in rice cereal for infants and young children ranged from 97.7%to 106.9%with the relative standard deviation(RSD) of 1.7%(n=6). The sensitivity,accuracy and precision of the method can meet the technical standards requirement of the aflatoxin B1 determination. Therefore the method can be applied to the determination of the aflatoxin B1 in rice cereal for infants and young children.