CAJ | 학술논문

从发酵蔬菜中分离到乳杆菌DMDL 9010，将此菌与LCR 719、LB 1．83、ST 1．204和LP 8140等4株乳酸菌用于MRS培养基体系中亚硝酸盐的降解，作用24 h后发现，DM-DL 9010的降解效果最好并具有显著性（P≤0．001）．利用16 S rDNA将鉴别的范围缩小到植物乳杆菌（Lactobacillus plantarum）或戊糖乳杆菌（Lactobacillus pentosus），生理生化实验将DMDL 9010鉴定为戊糖乳杆菌．但在后续的PCR中无法得到戊糖乳杆菌的特异性条带．通过分析两种菌的基因组序列，发现它们都具有编码L-乳酸脱氢酶1的同源DNA序列（ldhL1），序列相似度达到90％．同时这段同源序列上下游片段序列相似度较低，其序列的差异可以作为鉴别依据．对DMDL 9010的L-乳酸脱氢酶1基因上下游900 bp左右的DNA片段进行PCR扩增并测序，利用生物信息学分析方法进行序列比对分析，DMDL 9010被鉴定为植物乳杆菌．
종발효소채중분리도유간균DMDL 9010，장차균여LCR 719、LB 1．83、ST 1．204화LP 8140등4주유산균용우MRS배양기체계중아초산염적강해，작용24 h후발현，DM-DL 9010적강해효과최호병구유현저성（P≤0．001）．이용16 S rDNA장감별적범위축소도식물유간균（Lactobacillus plantarum）혹무당유간균（Lactobacillus pentosus），생리생화실험장DMDL 9010감정위무당유간균．단재후속적PCR중무법득도무당유간균적특이성조대．통과분석량충균적기인조서렬，발현타문도구유편마L-유산탈경매1적동원DNA서렬（ldhL1），서렬상사도체도90％．동시저단동원서렬상하유편단서렬상사도교저，기서렬적차이가이작위감별의거．대DMDL 9010적L-유산탈경매1기인상하유900 bp좌우적DNA편단진행PCR확증병측서，이용생물신식학분석방법진행서렬비대분석，DMDL 9010피감정위식물유간균．
Lactobacillus sp. DMDL 9010 was isolated from fermented vegetables and was used for the degradation of nitrites in MRS broth,and the results are compared with those of other four lactic acid bacteria including LCR 719, LB 1 . 83 ,ST 1 . 204 and LP 8140 ,with the best nitrite degradation effect of DMDL 9010 after 24-h fermentation (P≤0. 001)being revealed. Then,the identification scope of DMDL 9010 was reduced to Lactobacillus plantarum and Lactobacillus pentosus with the help of 16S rDNA sequence,and the strain was identified as L. pentosus by tes-ting physiological and biochemical characters,although no specific stripe was obtained in the subsequent PCR. More-over,a homologous DNA sequence of L-lactate dehydrogenase 1 (ldhL1)with a similarity of 90% was found in the genome of L. pentosus and L. plantarum,and the low DNA sequence similarity in the upstream and downstream fragments of ldhL1 could be regarded as an identification criterion of the two strains. Finally,after the PCR amplifi-cation and sequencing of the upstream and downstream fragments of ldhL1 ,the above-mentioned sequences were compared via the bioinformatics analysis,and DMDL 9010 was identified as L. plantarum.