华南理工大学学报（自然科学版）
華南理工大學學報（自然科學版）
화남리공대학학보（자연과학판）
JOURNAL OF SOUTH CHINA UNIVERSITY OF TECHNOLOGY(NATURAL SCIENCE EDITION)
2014年
3期
125-130
,共6页
模拟体外消化%ACE抑制活性%大豆多肽%酶促水解
模擬體外消化%ACE抑製活性%大豆多肽%酶促水解
모의체외소화%ACE억제활성%대두다태%매촉수해
simulated in-vitro digestion%ACE inhibitory activity%soybean peptide%enzymatic hydrolysis
为了验证大豆多肽血管紧张素转换酶（ACE）抑制活性的生物可获得性，采用Al-calase蛋白酶催化大豆蛋白水解获得了3种水解程度不同的大豆多肽，再应用摸拟体外消化方法处理大豆蛋白及其水解产物，并进一步分析了摸拟消化前后大豆多肽结构、性质和ACE抑制活性的变化。结果显示：3个大豆多肽的相对分子质量均小于16000，且水解度越高，TCA可溶性蛋白含量就越高；经过摸拟体外消化，大豆蛋白出现降解，其分子大小和性质与大豆多肽趋于一致，大豆多肽则进一步降解；3种大豆多肽的ACE抑制活性在经过摸拟体外消化后显著下降，大豆蛋白的ACE抑制活性则显著上升，两者之间没有显著差异，表明虽然Alcalase对大豆蛋白的酶促处理能够释放降血压肽，但经过模拟体外消化后其ACE抑制活性又会下降，而直接口服大豆蛋白有可能获得同样的降血压效果。
為瞭驗證大豆多肽血管緊張素轉換酶（ACE）抑製活性的生物可穫得性，採用Al-calase蛋白酶催化大豆蛋白水解穫得瞭3種水解程度不同的大豆多肽，再應用摸擬體外消化方法處理大豆蛋白及其水解產物，併進一步分析瞭摸擬消化前後大豆多肽結構、性質和ACE抑製活性的變化。結果顯示：3箇大豆多肽的相對分子質量均小于16000，且水解度越高，TCA可溶性蛋白含量就越高；經過摸擬體外消化，大豆蛋白齣現降解，其分子大小和性質與大豆多肽趨于一緻，大豆多肽則進一步降解；3種大豆多肽的ACE抑製活性在經過摸擬體外消化後顯著下降，大豆蛋白的ACE抑製活性則顯著上升，兩者之間沒有顯著差異，錶明雖然Alcalase對大豆蛋白的酶促處理能夠釋放降血壓肽，但經過模擬體外消化後其ACE抑製活性又會下降，而直接口服大豆蛋白有可能穫得同樣的降血壓效果。
위료험증대두다태혈관긴장소전환매（ACE）억제활성적생물가획득성，채용Al-calase단백매최화대두단백수해획득료3충수해정도불동적대두다태，재응용모의체외소화방법처리대두단백급기수해산물，병진일보분석료모의소화전후대두다태결구、성질화ACE억제활성적변화。결과현시：3개대두다태적상대분자질량균소우16000，차수해도월고，TCA가용성단백함량취월고；경과모의체외소화，대두단백출현강해，기분자대소화성질여대두다태추우일치，대두다태칙진일보강해；3충대두다태적ACE억제활성재경과모의체외소화후현저하강，대두단백적ACE억제활성칙현저상승，량자지간몰유현저차이，표명수연Alcalase대대두단백적매촉처리능구석방강혈압태，단경과모의체외소화후기ACE억제활성우회하강，이직접구복대두단백유가능획득동양적강혈압효과。
In order to verify the bioavailability of ACE (Angiotensin Converting Enzyme )inhibitory activities of soybean peptides,3 kinds of soybean peptides with different hydrolysis degrees were prepared by using Alcalase protease to catalyze the hydrolysis of soybean protein,and the effects of simulated in-vitro digestion on the struc-ture,properties and ACE inhibitory activities of soybean protein isolates (SPI)and soybean peptides were ana-lyzed. The results show that (1)the relative molecular mass of the three kinds of soybean peptides are all less than 16 000 ,and the content of TCA soluble protein increases with the degree of hydrolysis;(2 )after the simulated in-vitro digestion,SPI degrades,with its molecular size and properties tending to those of soybean peptides,and,at the same time,soybean peptides further degrade;and (3)there is no significant difference between SPI and soy-bean peptides because the simulated in-vitro digestion results in a significant decrease of ACE inhibitory activities of soybean peptides and a remarkable increase of those of SPI. It is thus concluded that SPI is able to release antihy-pertensive peptides under the action of Alacalase protease,but the ACE inhibitory activities of soybean peptides may reduce after the simulated in-vitro digestion;and that SPI by oral administration may have the same effect of lowering blood pressure.
