CAJ | 학술논문

目的：探讨LRIG3反义寡核苷酸（ ASODN）对宫颈鳞癌Hela229细胞LRIG3、EGFR表达及增殖的影响。方法：利用阳离子脂质体介导法分别用150、200及250 mg/L的LRIG3 ASODN、正义寡核苷酸（SODN）及无关序列核苷酸（ MSODN）转染Hela229细胞24、48及72 h，分别应用RT-PCR和Western blot法检测细胞LRIG3、EGFR mR-NA及蛋白的表达情况，应用MTT法检测细胞增殖率。以常规培养的细胞作空白对照。结果：转染24、48、72 h后， ASODN组细胞增殖率均较空白对照组、SODN组、MSODN组明显增高（P＜0．05），LRIG3 mRNA和蛋白表达降低（P＜0．05），EGFR mRNA和蛋白表达增高（P＜0．05）；3个ASODN剂量组间比较，随着LRIG3 ASODN剂量的增加，细胞增殖率增高（P＜0．05），LRIG3 mRNA和蛋白表达水平降低（P＜0．05），EGFR mRNA和蛋白表达水平升高（P＜0．05）。结论：LRIG3 ASODN可促进宫颈鳞癌Hela229细胞的增殖，该效应可能与抑制LRIG3表达、促进EG-FR表达有关。
목적：탐토LRIG3반의과핵감산（ ASODN）대궁경린암Hela229세포LRIG3、EGFR표체급증식적영향。방법：이용양리자지질체개도법분별용150、200급250 mg/L적LRIG3 ASODN、정의과핵감산（SODN）급무관서렬핵감산（ MSODN）전염Hela229세포24、48급72 h，분별응용RT-PCR화Western blot법검측세포LRIG3、EGFR mR-NA급단백적표체정황，응용MTT법검측세포증식솔。이상규배양적세포작공백대조。결과：전염24、48、72 h후， ASODN조세포증식솔균교공백대조조、SODN조、MSODN조명현증고（P＜0．05），LRIG3 mRNA화단백표체강저（P＜0．05），EGFR mRNA화단백표체증고（P＜0．05）；3개ASODN제량조간비교，수착LRIG3 ASODN제량적증가，세포증식솔증고（P＜0．05），LRIG3 mRNA화단백표체수평강저（P＜0．05），EGFR mRNA화단백표체수평승고（P＜0．05）。결론：LRIG3 ASODN가촉진궁경린암Hela229세포적증식，해효응가능여억제LRIG3표체、촉진EG-FR표체유관。
Aim:To observe the proliferation and expressions of LRIG 3 and EGFR in Hela229 cells transfected with LRIG3-targeted antisense oligonucleotide (ASODN).Methods:The ASODN, SODN and MSODN of LRIG3 at doses of 150, 200 and 250 mg/L were transfected into Hela229 cells by the cationic liposome respectively for 24, 48, and 72 h. RT-PCR and Western blot were used to detect the expressions of LRIG 3 and EGFR,and MTT assay was used to examine the proliferation of Hela229 cells.The cells with normal incubation were the blank control .Results:Compared with the blank control group, SODN group and MSODN group, the proliferation rate of ASODN group was significantly higher (P<0.05), the expressions of LRIG3 mRNA and protein decreased(P<0.05), and the expressions of EGFR mRNA and protein in-creased with the increase of ASODN concentration ( P<0 .05 ) .Conclusion: LRIG3 ASODN can promote the proliferation of Hela229 cells, which is related to inhibition of LRIG 3 expression and promotion of EGFR expression .