郑州大学学报(医学版)
鄭州大學學報(醫學版)
정주대학학보(의학판)
JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES)
2014年
3期
312-314,315
,共4页
余文发%赵玉林%王萍%鲁保才%马慧敏%王慧敏
餘文髮%趙玉林%王萍%魯保纔%馬慧敏%王慧敏
여문발%조옥림%왕평%로보재%마혜민%왕혜민
喉肿瘤%氯离子通道%Oct4%CD133%裸鼠%Hep-2
喉腫瘤%氯離子通道%Oct4%CD133%裸鼠%Hep-2
후종류%록리자통도%Oct4%CD133%라서%Hep-2
laryngeal neoplasm%chloride ion channel%Oct4%CD133%nude mouse%Hep-2
目的:研究阻断氯离子通道对荷Hep-2细胞瘤裸鼠移植瘤的抑制作用及可能机制。方法:以Hep-2细胞建立裸鼠皮下移植瘤模型,应用不同浓度(0、10、50、100和150μmol/L)的氯离子通道阻断剂( NPPB)处理4周,每组6只。观察移植瘤体积的变化,采用TUNEL染色检测移植瘤组织细胞凋亡情况,免疫组化法检测移植瘤组织Oct4和CD133蛋白的表达。结果:不同浓度的NPPB组间移植瘤体积、细胞凋亡指数及Oct4和CD133蛋白的阳性表达率差异均有统计学意义(F=7.361、126.385,P均<0.05);与对照组(0μmol/L NPPB组)相比,50、100和150μmol/L NPPB组移植瘤体积减小,细胞凋亡指数升高,Oct4和CD133蛋白的阳性表达率降低(P<0.05或0.005)。结论:体内阻断氯离子通道可抑制荷Hep-2细胞瘤裸鼠移植瘤的生长,诱导肿瘤细胞凋亡,其机制可能与降低Oct4和CD133蛋白的表达有关。
目的:研究阻斷氯離子通道對荷Hep-2細胞瘤裸鼠移植瘤的抑製作用及可能機製。方法:以Hep-2細胞建立裸鼠皮下移植瘤模型,應用不同濃度(0、10、50、100和150μmol/L)的氯離子通道阻斷劑( NPPB)處理4週,每組6隻。觀察移植瘤體積的變化,採用TUNEL染色檢測移植瘤組織細胞凋亡情況,免疫組化法檢測移植瘤組織Oct4和CD133蛋白的錶達。結果:不同濃度的NPPB組間移植瘤體積、細胞凋亡指數及Oct4和CD133蛋白的暘性錶達率差異均有統計學意義(F=7.361、126.385,P均<0.05);與對照組(0μmol/L NPPB組)相比,50、100和150μmol/L NPPB組移植瘤體積減小,細胞凋亡指數升高,Oct4和CD133蛋白的暘性錶達率降低(P<0.05或0.005)。結論:體內阻斷氯離子通道可抑製荷Hep-2細胞瘤裸鼠移植瘤的生長,誘導腫瘤細胞凋亡,其機製可能與降低Oct4和CD133蛋白的錶達有關。
목적:연구조단록리자통도대하Hep-2세포류라서이식류적억제작용급가능궤제。방법:이Hep-2세포건립라서피하이식류모형,응용불동농도(0、10、50、100화150μmol/L)적록리자통도조단제( NPPB)처리4주,매조6지。관찰이식류체적적변화,채용TUNEL염색검측이식류조직세포조망정황,면역조화법검측이식류조직Oct4화CD133단백적표체。결과:불동농도적NPPB조간이식류체적、세포조망지수급Oct4화CD133단백적양성표체솔차이균유통계학의의(F=7.361、126.385,P균<0.05);여대조조(0μmol/L NPPB조)상비,50、100화150μmol/L NPPB조이식류체적감소,세포조망지수승고,Oct4화CD133단백적양성표체솔강저(P<0.05혹0.005)。결론:체내조단록리자통도가억제하Hep-2세포류라서이식류적생장,유도종류세포조망,기궤제가능여강저Oct4화CD133단백적표체유관。
Aim:To study the effects of blocking chloride ion channel on human laryngeal carcinoma xenograft tumor in nude mice and its possible mechanism .Methods:Hep-2 cells were seeded subcutaneously into the nude mice to estab-lish human laryngeal carcinoma xenograft tumor model and treated with NPPB at different concentrations (0,10,50,100 and 150 μmol/L), respectively for 4 weeks.The volume changes of the xenograft tumors were studied .TUNEL staining was used to determine cell apoptosis in xenograft tumor tissue .Immunohistochemistry staining was used to detect the expressions of Oct4 and CD133 .Results:The volume of the tumors , apoptosis index , and the positive expression rates of Oct 4 and CD133 in the 5 groups had significant differences (F=7.361,126.385,P<0.05);compared with 0μmol/L NPPB group, the volume of the tumors decreased , the apoptosis index increased , and the positive expression rates of Oct 4 and CD133 de-creased in 50,100,and 150 μmol/L NPPB groups(P<0.05 or 0.005).Conclusion: Blocking the chloride ion channel may inhibit the growth of xenograft tumor , induce apoptosis of Hep-2 cells in the xenograft tumors through down-regulating the expressions of Oct 4 and CD133 .