CAJ | 학술논문

目的：探讨河南省间日疟原虫环子孢子蛋白（ CSP ）的基因型分布。方法：收集经镜检确诊的河南省2011年间日疟患者血标本34份，24例患者为河南籍，发病前无外出到其他省份或国家的历史，10例患者有明确外出史。用核酸提取试剂盒提取滤纸血中疟原虫DNA，进行巢式PCR扩增，对扩增产物进行序列测定和基因进化特征分析。结果：24份本地病例分离所得的本地株间日疟原虫CSP均属于PV-Ⅰ型，又可分为A和B 2个基因型；氨基酸同源性分析结果显示，A型与VK210的同源性达90．6％～94．2％，B型为71．6％。结论：河南省间日疟原虫CSP的基因型均属PV-Ⅰ型，可以进一步分为2个基因型，与其他国家的基因型显著不同。
목적：탐토하남성간일학원충배자포자단백（ CSP ）적기인형분포。방법：수집경경검학진적하남성2011년간일학환자혈표본34빈，24례환자위하남적，발병전무외출도기타성빈혹국가적역사，10례환자유명학외출사。용핵산제취시제합제취려지혈중학원충DNA，진행소식PCR확증，대확증산물진행서렬측정화기인진화특정분석。결과：24빈본지병례분리소득적본지주간일학원충CSP균속우PV-Ⅰ형，우가분위A화B 2개기인형；안기산동원성분석결과현시，A형여VK210적동원성체90．6％～94．2％，B형위71．6％。결론：하남성간일학원충CSP적기인형균속PV-Ⅰ형，가이진일보분위2개기인형，여기타국가적기인형현저불동。
Aim:To explore circumsporozoite protein (CSP) genotypes structure of Plasmodium vivax(P.vivax) in Henan.Methods:Blood samples of 34 malaria patients from Henan Province in 2011 were collected to extract DNA ,and amplified using nested PCR technique , and the product was sequenced and analyzed .Results:The results showed that the 24 specimens from Henan indigenous cases all belonged to PV-Ⅰ;amino acid homology analysis showed that the specimens could be divided into two genotypes:A and B, the sequence of type A showed 90.6%-94.2%homology to VK210, while type B showed 71 .6%.Conclusion:The P.vivax CSP genotype of Henan all belongs to PV-Ⅰ, and it can be further di-vided into two genotypes , which is significantly different from other countries .