CAJ | 학술논문

目的：研究17aα-D-高炔雌二醇-3-乙酯（DHEA）对137Csγ射线和环磷酰胺（CTX）所致小鼠造血及免疫功能损伤的保护作用。方法分别用8．0 Gy 137Csγ射线和CTX建立C57BL/6小鼠动物模型，观察DHEA低、中、高3个剂量对两个模型小鼠的造血干细胞脾结节形成细胞（CFU-S）数、外周血WBC、骨髓有核细胞数、脾脏指数的影响。结果 DHEA各组对小鼠137Csγ射线照射所致的外周血WBC、CFU-S数、骨髓有核细胞数的降低具有保护作用，并增大了脾脏指数。DHEA各组对环磷酰胺引起的小鼠WBC、CFU-S数、骨髓有核细胞数降低有抑制作用。结论 DHEA能减轻辐射和环磷酰胺对小鼠造血系统的损伤。
목적：연구17aα-D-고결자이순-3-을지（DHEA）대137Csγ사선화배린선알（CTX）소치소서조혈급면역공능손상적보호작용。방법분별용8．0 Gy 137Csγ사선화CTX건립C57BL/6소서동물모형，관찰DHEA저、중、고3개제량대량개모형소서적조혈간세포비결절형성세포（CFU-S）수、외주혈WBC、골수유핵세포수、비장지수적영향。결과 DHEA각조대소서137Csγ사선조사소치적외주혈WBC、CFU-S수、골수유핵세포수적강저구유보호작용，병증대료비장지수。DHEA각조대배린선알인기적소서WBC、CFU-S수、골수유핵세포수강저유억제작용。결론 DHEA능감경복사화배린선알대소서조혈계통적손상。
Objective To observe the protective function of dehydroepiandrosterone (DHEA) on hematopoietic in C57BL/6 mouse induced by radiotherapy and chemotherapy. Methods Constructing the animal models with hematopoietic injury by 8.0 Gy 137Csγray and cyclophosphamide respectively. Observe the changes of colony-forming unit-spleen(CFU-S), populations of peripheral WBC and bone marrow nu-cleated cells and index of spleen in all DHEA treated groups. Results DHEA can reduce injury of 137Csγray and cyclophosphamide induced hematopoietic function injury of C57BL/6 mouse. Compared with the blank control group, the DHEA group can significantly increase the number of peripheral blood leukocyte, bone marrow nucleated cells and CFU-S, and there is statistically difference between DHEA treated group and the blank control group. Index of spleen in the high dose group is higher than that in the blank control group. Conclusion DHEA have protection function on hematopoietic in C57BL/6 mouse induced by ra-diotherapy and chemotherapy.