CAJ | 학술논문

雄性SD大鼠气管滴注不同剂量的纳米二氧化钛（TiO2），每周2次，连续4周，染毒结束后，检测大鼠血常规、肺组织氧化应激指标和CRP表达，观察肺组织病理学和超微结构改变。结果表明：纳米TiO232 mg/kg组血常规中NEUT计数增加，纳米TiO2引起肺组织中SOD，GSH和CAT含量降低，组织病理学显示随着纳米TiO2染毒剂量增加，肺泡扩张不良、闭塞比例增加，肺内吞噬棕黄色颗粒的巨噬细胞数量增加，肺泡间隔内淋巴细胞和纤维细胞增多；超微结构发现纳米TiO2主要引起Ⅱ型肺泡结构改变。结果显示，在本实验条件下纳米TiO2使大鼠的肺组织受到氧自由基的攻击，肺脏清除氧自由基的能力减弱，并引起肺组织不同程度的病理和超微结构的改变。
웅성SD대서기관적주불동제량적납미이양화태（TiO2），매주2차，련속4주，염독결속후，검측대서혈상규、폐조직양화응격지표화CRP표체，관찰폐조직병이학화초미결구개변。결과표명：납미TiO232 mg/kg조혈상규중NEUT계수증가，납미TiO2인기폐조직중SOD，GSH화CAT함량강저，조직병이학현시수착납미TiO2염독제량증가，폐포확장불량、폐새비례증가，폐내탄서종황색과립적거서세포수량증가，폐포간격내림파세포화섬유세포증다；초미결구발현납미TiO2주요인기Ⅱ형폐포결구개변。결과현시，재본실험조건하납미TiO2사대서적폐조직수도양자유기적공격，폐장청제양자유기적능력감약，병인기폐조직불동정도적병리화초미결구적개변。
Sprague Dawley rats were exposed to intra-tracheal instillation of nano-TiO2 with different doses.The exposure was conducted twice a week,for four consecutive weeks.After four weeks, the subacute pulmonary toxicity was assessed by the hematological parameters,enzyme activities,C-reactive protein (CRP ) level, pathological examination and transmission electron microscopy (TEM).Results show that the neutrophil count (NEUT)increased in the group of 32 mg/kg nano-TiO2 and the content of hydrogen peroxide (CAT),glutathione (GSH)and superoxide dismutase (SOD)activities decreased.The results of lung histology demonstrate the expansion of lung gaps and slight alveolar thickness,increased proliferation of tissue macrophages of deposition massive par-ticulate,increased lymphocytes and fibrocyte with the increase of nano TiO2 dose.Type II cells have prominent lamellar bodies and the damage of lamellar bodies are observed by TEM.Nano-TiO2 can induce the change of enzyme activities and histology,and present inflammation and massive particu-late deposition in pulmonary tissues and pulmonary injury.