CAJ | 학술논문

为了探索锥形纳米孔整流的机理，以硼硅酸盐玻璃毛细管为材料，采用微电极拉制仪P 2000拉制了内径20～50 nm的玻璃锥形纳米孔。孔内注满氯化钠盐溶液后，采用膜片钳对该类纳米孔的整流效应进行了实验研究。实验发现，锥形玻璃纳米孔的整流比随浓度先增大后减小，在溶液浓度为10～50 mmol/L范围内出现最大整流比。当溶液pH值高于玻璃等电点时，随着溶液pH值的增大，整流比随之增大；当溶液pH值低于玻璃等电点时，玻璃表面发生壁面电荷倒置，整流方向发生改变。实验结果表明，玻璃锥形纳米孔整流效应受到溶液浓度和溶液pH值共同影响，纳米孔内壁不经过任何化学修饰就可以发生电荷倒置。
위료탐색추형납미공정류적궤리，이붕규산염파리모세관위재료，채용미전겁랍제의P 2000랍제료내경20～50 nm적파리추형납미공。공내주만록화납염용액후，채용막편겸대해류납미공적정류효응진행료실험연구。실험발현，추형파리납미공적정류비수농도선증대후감소，재용액농도위10～50 mmol/L범위내출현최대정류비。당용액pH치고우파리등전점시，수착용액pH치적증대，정류비수지증대；당용액pH치저우파리등전점시，파리표면발생벽면전하도치，정류방향발생개변。실험결과표명，파리추형납미공정류효응수도용액농도화용액pH치공동영향，납미공내벽불경과임하화학수식취가이발생전하도치。
In order to explore the mechanism of the conical nanopore rectification,glass conical nan-opores were fabricated with boron silicate glass capillary on micropipette puller system P 2000.The nanopores' diameter ranged from 20 to 50 nm.After the pore was filled with sodium chloride solu-tion,the rectification effect of the pore was experimentally studied with a patch clamp.It is found that the rectification ratio of the nanopore shows an increase and then decrease trend as the solution concentration becomes higher,and the highest rectification ratio appears when the salt concentration is between 10 and 50 mmol/L.When the pH is above the isoelectric point of glass,the rectification ratio increases as the solution pH increases.When the pH is below the isoelectric point of glass,the surface of the glass exhibits charge inversion and the rectification direction changes.The results show that conical nanopore rectification can be influenced both by salt concentration and pH value,and charge inversion can occur on the surface of glass without any chemical modification.