CAJ | 학술논문

RAV 转录因子是 AP2/ERF 家族的成员之一，在植物生长发育和逆境调控中起着重要作用。本研究以安吉白茶和迎霜这两个茶树（Camellia sinensis）品种为试验材料，通过 PCR 和 RT-PCR 方法分别从两种茶树的DNA和cDNA中克隆得到CsRAV2基因。分析显示，来源于两种茶树中的CsRAV2基因全长均为1089 bp，没有内含子，分别编码362个氨基酸，含有相对保守的AP2结合域和B3结构域，具有典型的植物RAV类转录因子特征。从氨基酸组成成分、理化性质、亲水性/疏水性、三级结构上分析显示，茶树中 CsRAV2转录因子是亲水性蛋白。茶树中 CsRAV2转录因子与拟南芥 AtRAV 具有相似的三级结构。实时定量 PCR分析表明，茶树中CsRAV2基因在茶树根中表达量最高，高温、低温、NaCl处理均能诱导该基因表达，不同品种间存在差异。
RAV 전록인자시 AP2/ERF 가족적성원지일，재식물생장발육화역경조공중기착중요작용。본연구이안길백다화영상저량개다수（Camellia sinensis）품충위시험재료，통과 PCR 화 RT-PCR 방법분별종량충다수적DNA화cDNA중극륭득도CsRAV2기인。분석현시，래원우량충다수중적CsRAV2기인전장균위1089 bp，몰유내함자，분별편마362개안기산，함유상대보수적AP2결합역화B3결구역，구유전형적식물RAV류전록인자특정。종안기산조성성분、이화성질、친수성/소수성、삼급결구상분석현시，다수중 CsRAV2전록인자시친수성단백。다수중 CsRAV2전록인자여의남개 AtRAV 구유상사적삼급결구。실시정량 PCR분석표명，다수중CsRAV2기인재다수근중표체량최고，고온、저온、NaCl처리균능유도해기인표체，불동품충간존재차이。
The RAV transcription factor, one subfamily of AP2/ERF family transcription factor, includes several genes that encode proteins involved in the development and regulation of abiotic/biotic resistance in higher plant. The CsRAV2 genes, which encoding to the RAV transcription factor, were cloned from tea plant ( Camellia sinensis) cultivars‘Anjibaicha’ and ‘Yingshuang’ by PCR and RT-PCR using DNA and cDNA as template, respectively. Then, nucleic acid and deduced amino acid sequence, phylogenetic tree, and molecular modeling were predicted and analyzed. The lengths of CsRAV2 genes from the two tea plant cultivars were 1 089 bp, encoding 362 amino acids. No intron was found in the CsRAV2 gene. The transcription factor of CsRAV2 contained two distinct DNA domains mainly found in higher plants RAV family factors, one AP2 domain together with one B3 domain. The CsRAV2 were hydrophilic protein. The protein of CsRAV2 from tea plant and AtRAV from Arabidopsis had similar three-dimension structure. Quantitative real-time PCR analysis of the expression profiles showed that the CsRAV2 gene was tissue-specific expressed in the two tea plant cultivars. The highest expression levels of the CsRAV2 gene were found in the root. The CsRAV2 gene was induced by high temperature, low temperature, PEG and high-salinity treatment, respectively. There were differences profiles between different teacultivars.