CAJ | 학술논문

采用气相色谱/质谱联用（GC-MS）技术，分别应用正构烷烃（n-alkanes）混标和分析物中的正构烷烃对茶叶保留指数（RI）进行了测定，得到的保留时间相对碳数的线性相关系数（R2）均超过了0.99，证实了两种保留指数测定方法的可靠性。在谱库检索的基础上结合保留指数法，龙井茶香气成分的鉴定率从46.67%提高到74.67%，大多数物质可得到完全鉴定。同时，保留指数能对质谱匹配过程中存在的同分异构体进行有效的鉴定，提高物质定性的准确性，使物质鉴定效率大大提高。归纳相关文献数据，构建了HP-innowax色谱柱的保留指数库，提高了利用HP-innowax色谱柱进行挥发物分析的效率。
채용기상색보/질보련용（GC-MS）기술，분별응용정구완경（n-alkanes）혼표화분석물중적정구완경대다협보류지수（RI）진행료측정，득도적보류시간상대탄수적선성상관계수（R2）균초과료0.99，증실료량충보류지수측정방법적가고성。재보고검색적기출상결합보류지수법，룡정다향기성분적감정솔종46.67%제고도74.67%，대다수물질가득도완전감정。동시，보류지수능대질보필배과정중존재적동분이구체진행유효적감정，제고물질정성적준학성，사물질감정효솔대대제고。귀납상관문헌수거，구건료HP-innowax색보주적보류지수고，제고료이용HP-innowax색보주진행휘발물분석적효솔。
Standard mixture of n-alkanes and n-alkanes in samples were applied respectively to determine the retention index (RI) of tea volatiles. The linear correlation coefficients of retention time with carbon number for the two methods were both above 0.99, indicating quite good reliability for calculating RI. With the applying of RI, the correct identification rate of the volatiles increased from 46.67% to 74.67%. Meanwhile, RI enabled effective identification of the isomers contained in tea volatiles, and greatly increased the identification accuracy. Data in relevant literatures were summarized to construct a RI database for HP-innowax column. The RI database could also improve the efficiency of compound identification using HP-innowax column.