CAJ | 학술논문

研究了表没食子儿茶素没食子酸酯（EGCG）和茶氨酸两种茶叶主要活性成分单独和协同清除2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐（ABTS）、1,1-二苯基-2-三硝基苯肼（DPPH）和羟自由基的效果，并通过建立细胞氧化损伤模型，测定两种化合物对氧化损伤细胞的氧化水平和抗氧化酶活性的影响。结果表明：EGCG和茶氨酸对ABTS和羟自由基的清除有协同增效作用，对DPPH自由基无协同作用；在细胞水平， EGCG 和茶氨酸可协同降低细胞内活性氧自由基及脂质过氧化水平，提高谷胱甘肽过氧化物酶的活性，即两者共同作用可协同降低细胞所受的氧化损伤，提高细胞抗氧化能力。
연구료표몰식자인다소몰식자산지（EGCG）화다안산량충다협주요활성성분단독화협동청제2,2-련담-이(3-을기-분병새서-6-광산)이안염（ABTS）、1,1-이분기-2-삼초기분정（DPPH）화간자유기적효과，병통과건립세포양화손상모형，측정량충화합물대양화손상세포적양화수평화항양화매활성적영향。결과표명：EGCG화다안산대ABTS화간자유기적청제유협동증효작용，대DPPH자유기무협동작용；재세포수평， EGCG 화다안산가협동강저세포내활성양자유기급지질과양화수평，제고곡광감태과양화물매적활성，즉량자공동작용가협동강저세포소수적양화손상，제고세포항양화능력。
This paper investigated the scavenging capacities of EGCG and theanine , the two main active components in tea, alone or synergistically on ABTS, DPPH and hydroxyl radicals in vitro were investigated. The oxidative injury experimental model of mouse macrophage (RAW 264.7) by H2O2 was established for measuring the oxidative level and antioxidative enzyme activities by EGCG and theanine. The results showed that EGCG and theanine showed synergistic effect on the scavenging of ABTS and hydroxyl radical except for the DPPH radical. EGCG and theanine could synergistically reduce the contents of reactive oxygen species and lipid peroxidation, and enhance the activity of GSH-Px on RAW 264.7, which meant that the combination of above two compounds could synergistically reduce the oxidative damage of the cells through improving the antioxidant capacity.