茶叶科学
茶葉科學
다협과학
2014年
3期
230-238
,共9页
滕翠琴%刘仲华%龚受基%彭雨轩%马蕊
滕翠琴%劉仲華%龔受基%彭雨軒%馬蕊
등취금%류중화%공수기%팽우헌%마예
六堡茶%糖脂代谢%胰岛素抵抗%3T3-L1脂肪细胞
六堡茶%糖脂代謝%胰島素牴抗%3T3-L1脂肪細胞
륙보다%당지대사%이도소저항%3T3-L1지방세포
Liupao tea%glucolipid metabolism%insulin resistance%3T3-L1 adipocytes
采用软脂酸诱导3T3-L1脂肪细胞胰岛素抵抗后,给予不同剂量的六堡茶水提物治疗,探讨六堡茶对胰岛素抵抗3T3-L1脂肪细胞的糖脂代谢的影响。结果表明,1 mmol·L-1的软脂酸对3T3-L1脂肪细胞作用24 h后,葡萄糖摄取量减少,建立胰岛素抵抗模型。六堡茶对模型细胞干预24 h 后,与模型组比较,细胞培养基的残留液中葡萄糖和游离脂肪酸的含量降低;对糖脂代谢相关关键酶(己糖激酶、6-磷酸果糖激酶-1、丙酮酸激酶、甘油-3-磷酸酰基转移酶)和葡萄糖转运子基因表达都有上调作用,并下调乙酰辅酶 A 羧化酶、蛋白酪氨酸磷酸酯酶1B基因表达。低剂量的六堡茶下调肉碱脂酰转移酶-I基因的表达,但是高剂量能显著上调肉碱脂酰转移酶-I基因表达。实验证明了六堡茶能够提高胰岛素抵抗3T3-L1脂肪细胞对葡萄糖的摄取能力,同时促进细胞内糖脂代谢,六堡茶有改善3T3-L1脂肪细胞胰岛素抵抗的作用。
採用軟脂痠誘導3T3-L1脂肪細胞胰島素牴抗後,給予不同劑量的六堡茶水提物治療,探討六堡茶對胰島素牴抗3T3-L1脂肪細胞的糖脂代謝的影響。結果錶明,1 mmol·L-1的軟脂痠對3T3-L1脂肪細胞作用24 h後,葡萄糖攝取量減少,建立胰島素牴抗模型。六堡茶對模型細胞榦預24 h 後,與模型組比較,細胞培養基的殘留液中葡萄糖和遊離脂肪痠的含量降低;對糖脂代謝相關關鍵酶(己糖激酶、6-燐痠果糖激酶-1、丙酮痠激酶、甘油-3-燐痠酰基轉移酶)和葡萄糖轉運子基因錶達都有上調作用,併下調乙酰輔酶 A 羧化酶、蛋白酪氨痠燐痠酯酶1B基因錶達。低劑量的六堡茶下調肉堿脂酰轉移酶-I基因的錶達,但是高劑量能顯著上調肉堿脂酰轉移酶-I基因錶達。實驗證明瞭六堡茶能夠提高胰島素牴抗3T3-L1脂肪細胞對葡萄糖的攝取能力,同時促進細胞內糖脂代謝,六堡茶有改善3T3-L1脂肪細胞胰島素牴抗的作用。
채용연지산유도3T3-L1지방세포이도소저항후,급여불동제량적륙보다수제물치료,탐토륙보다대이도소저항3T3-L1지방세포적당지대사적영향。결과표명,1 mmol·L-1적연지산대3T3-L1지방세포작용24 h후,포도당섭취량감소,건립이도소저항모형。륙보다대모형세포간예24 h 후,여모형조비교,세포배양기적잔류액중포도당화유리지방산적함량강저;대당지대사상관관건매(기당격매、6-린산과당격매-1、병동산격매、감유-3-린산선기전이매)화포도당전운자기인표체도유상조작용,병하조을선보매 A 최화매、단백락안산린산지매1B기인표체。저제량적륙보다하조육감지선전이매-I기인적표체,단시고제량능현저상조육감지선전이매-I기인표체。실험증명료륙보다능구제고이도소저항3T3-L1지방세포대포도당적섭취능력,동시촉진세포내당지대사,륙보다유개선3T3-L1지방세포이도소저항적작용。
To investigate the effect of Liupao tea on glucose and lipid metabolism in palmitate-induced insulin resistance 3T3-L1 adipocytes. Glucose intake decreased and insulin resistance was induced in 3T3-L1 adipocytes after incubation in 1mmol/L palmitate for 24 hours. Compared with the model group, the concentrations of glucose and NEFA decreased along with the increase of Liupao tea concentrations in medium. The mRNA expression of key enzyme for glycolysis of glucose (Hexokinase, 6-phosphofructokinase-1, and pyruvatekinase) and the Lipid-Metabolism-Related Enzymes (Sn-glycerol-3-phosphate acyltransferase) significantly increased in the Liupao tea groups comparing with model sample. Meanwhile the glucose transporter 4 mRNA expression was also increased and the acetyl-CoA carboxylase, protein tyrosine phosphatase 1B mRNA expression was decreased. Low doses of Liupao tea reduced carnitine palmitoyl transferase I mRNA expression while high doses significantly raised it. Research proved that Liupao tea were able to increase glucose uptake and activate the glucolipid metabolic pathways.Liupao tea showed the effect of improving insulin resistance induced by palmitate in 3T3 -L1 adipocytes.