福建农业学报
福建農業學報
복건농업학보
FUJIAN JOURNAL OF AGRICULTURAL SCIENCES
2014年
4期
389-392
,共4页
鱼籽酱%盐酸环丙沙星%恩诺沙星%高效液相色谱法
魚籽醬%鹽痠環丙沙星%恩諾沙星%高效液相色譜法
어자장%염산배병사성%은낙사성%고효액상색보법
Caviar%Ciprofloxacin Hydrochloride%Enrofloxacin%HPLC
建立了鱼籽酱中盐酸环丙沙星和恩诺沙星的高效液相色谱-荧光检测方法。样品经甲醇及酸化乙腈提取,提取液经氮吹浓缩,残渣用2 mL流动相溶解,正己烷脱脂净化,采用Eclipse XDB-C18色谱柱,以乙腈∶0.02 mol · L -1草酸(14∶86)为流动相,荧光检测器检测。盐酸环丙沙星和恩诺沙星的检出限分别为1、0.5μg · kg -1,在添加量5~120μg · kg -1范围内,平均回收率均大于80%,相对标准偏差均小于5%。说明该方法灵敏、准确,满足常规残留检测要求。
建立瞭魚籽醬中鹽痠環丙沙星和恩諾沙星的高效液相色譜-熒光檢測方法。樣品經甲醇及痠化乙腈提取,提取液經氮吹濃縮,殘渣用2 mL流動相溶解,正己烷脫脂淨化,採用Eclipse XDB-C18色譜柱,以乙腈∶0.02 mol · L -1草痠(14∶86)為流動相,熒光檢測器檢測。鹽痠環丙沙星和恩諾沙星的檢齣限分彆為1、0.5μg · kg -1,在添加量5~120μg · kg -1範圍內,平均迴收率均大于80%,相對標準偏差均小于5%。說明該方法靈敏、準確,滿足常規殘留檢測要求。
건립료어자장중염산배병사성화은낙사성적고효액상색보-형광검측방법。양품경갑순급산화을정제취,제취액경담취농축,잔사용2 mL류동상용해,정기완탈지정화,채용Eclipse XDB-C18색보주,이을정∶0.02 mol · L -1초산(14∶86)위류동상,형광검측기검측。염산배병사성화은낙사성적검출한분별위1、0.5μg · kg -1,재첨가량5~120μg · kg -1범위내,평균회수솔균대우80%,상대표준편차균소우5%。설명해방법령민、준학,만족상규잔류검측요구。
A method was developed for the determination of Ciprofloxacin Hydrochloride and Enrofloxacin in Caviar by high performance liquid chromatography with fluorescence detection . The homogenized sample was extracted with methanol and acidified acetonitrile .The extract was concentrated by nitrogen ,and redissolved into 2 mL mobile phase and defatted with n-hexane .Ciprofloxacin Hydrochloride and Enrofloxacin were separated on Eclipse XDB-C18 Column with mobile phases containing acetonitrile and 0.02 mol · L -1 oxalic acid (14 ∶ 86 ) and finally determined by fluorescence Detector .The detection limit of Ciprofloxacin Hydrochloride and Enrofloxacin were 1μg · kg -1 and 0.5 μg · kg -1 , separately .In the range of 5 -120 μg · kg -1 , the mean recoveries were more than 80% . The intraday and interday precisions were less than 5% .It showed that this accurate and sensitive method could be applied for the conventional residue testing requirements .