CAJ | 학술논문

选取均匀分布于水稻12条染色体上的134对SSR （Simple Sequence Repeats ，SSR）标记，对明恢63、明恢86、航1号和航2号等4个具有相似遗传背景的水稻骨干恢复系进行遗传多态性分析。结果表明，经航天诱变选育的“航1号、航2号”与原种明恢86之间存在不同程度的遗传多样性。在筛选的134对SSR引物中，具有多态性标记为39对，多态性频率为29.10％。每个标记位点的平均等位基因数为2.10个，变幅为1～4个，平均多态性信息含量指数（PIC ）为0.37。聚类分析结果表明，当以0.87为阀值时可将航天诱变选育的“航1号、航2号”与原种“明恢86”区分开。其中在标记RM234两侧邻近的7个含有重要基因区段的SSR标记中有5个标记存在多态性，表明明恢86在航天诱变过程中可能在具有重要生物学功能的基因座发生了突变，再经系统选育成航1号、航2号优良恢复系，为航天诱变中因发生有利变异而选育新品种提供分子证据。
선취균균분포우수도12조염색체상적134대SSR （Simple Sequence Repeats ，SSR）표기，대명회63、명회86、항1호화항2호등4개구유상사유전배경적수도골간회복계진행유전다태성분석。결과표명，경항천유변선육적“항1호、항2호”여원충명회86지간존재불동정도적유전다양성。재사선적134대SSR인물중，구유다태성표기위39대，다태성빈솔위29.10％。매개표기위점적평균등위기인수위2.10개，변폭위1～4개，평균다태성신식함량지수（PIC ）위0.37。취류분석결과표명，당이0.87위벌치시가장항천유변선육적“항1호、항2호”여원충“명회86”구분개。기중재표기RM234량측린근적7개함유중요기인구단적SSR표기중유5개표기존재다태성，표명명회86재항천유변과정중가능재구유중요생물학공능적기인좌발생료돌변，재경계통선육성항1호、항2호우량회복계，위항천유변중인발생유리변이이선육신품충제공분자증거。
The genetic evolution analysis of four mainly restore lines for hybrid rice including Minghui63 , Minghui86 ,Hang1 and Hang2 with similarly genetic background were carried out in the paper .A total of 134 pairs of simple sequence repeats (SSR) were selected distributed evenly from twelve chromosomes of rice (Oryzae sativa L .) .The results indicated that there were a lot of polymorphic loci between breeding varieties under space flight mutation (Hang1 ,Hang2) and original variety (Minghui86) ,39 polymorphic loci with a polymorphic ratio of 29.10% .The number of alleles per loci ranged from 1 to 4 with an average of 2.1. The value of polymorphism information content (PIC) was 0.37. The four mainly restore lines were classified into 2 groups by clustering .It is distinction between Hang 1 ,Hang2 and Minghui 86 when the threshold value is 0.87. A further analysis on SSR markers distributed in two sides of marker RM 234 which have key gene regions , indicated that there were 5 polymorphism sites from 7 pairs of SSR markers .Bioinformatics analysis showed that those loci have important biological function in Minghui 86 , was mutated under space flight mutation . Furthermore , breeding excellent restoring line Hang 1 and Hang 2 ,provided a molecular evidence that advantageous variations are in space flight mutation for rice breeding .