林业科学
林業科學
임업과학
SCIENTIA SILVAE SINICAE
2014年
4期
32-38
,共7页
鹅掌楸属%自交衰退%SSR%隐性致死基因
鵝掌楸屬%自交衰退%SSR%隱性緻死基因
아장추속%자교쇠퇴%SSR%은성치사기인
Liriodendron%selfing depression%SSR%recessive lethal gene
以鹅掌楸属树种5个自交组合子代为材料,以4个种内杂交子代及8个种间杂交子代为参照,以存活率、生长量等表型性状为评价指标,结合 SSR分子标记信息分析鹅掌楸属树种自交衰退程度,并试图从 SSR分子标记角度探究其遗传机制。结果表明,鹅掌楸属树种自交衰退明显。与种内异交及种间杂交子代相比,自交子代胸径、树高及存活率的衰退程度δ分别为:0.46,0.45;0.32,0.35;0.25,0.30。SSR 检测结果显示,不同交配类型子代的纯合子比率由高到低趋势为:自交、种内交配、种间交配。同时,初步筛选出2个可能与鹅掌楸属隐性致死基因相关联的 SSR位点。
以鵝掌楸屬樹種5箇自交組閤子代為材料,以4箇種內雜交子代及8箇種間雜交子代為參照,以存活率、生長量等錶型性狀為評價指標,結閤 SSR分子標記信息分析鵝掌楸屬樹種自交衰退程度,併試圖從 SSR分子標記角度探究其遺傳機製。結果錶明,鵝掌楸屬樹種自交衰退明顯。與種內異交及種間雜交子代相比,自交子代胸徑、樹高及存活率的衰退程度δ分彆為:0.46,0.45;0.32,0.35;0.25,0.30。SSR 檢測結果顯示,不同交配類型子代的純閤子比率由高到低趨勢為:自交、種內交配、種間交配。同時,初步篩選齣2箇可能與鵝掌楸屬隱性緻死基因相關聯的 SSR位點。
이아장추속수충5개자교조합자대위재료,이4개충내잡교자대급8개충간잡교자대위삼조,이존활솔、생장량등표형성상위평개지표,결합 SSR분자표기신식분석아장추속수충자교쇠퇴정도,병시도종 SSR분자표기각도탐구기유전궤제。결과표명,아장추속수충자교쇠퇴명현。여충내이교급충간잡교자대상비,자교자대흉경、수고급존활솔적쇠퇴정도δ분별위:0.46,0.45;0.32,0.35;0.25,0.30。SSR 검측결과현시,불동교배류형자대적순합자비솔유고도저추세위:자교、충내교배、충간교배。동시,초보사선출2개가능여아장추속은성치사기인상관련적 SSR위점。
The existing Liriodendron has a small population size,and hence easily occurs selfing depression. In this study,we analyzed selfing depression of Liriodendron by taking 5 selfing progenies as experimental populations,4 intra-species progenies and 8 inter-species progenies as reference populations. The phenotypic traits such as survival rate and growth were used as evaluation index,combined with SSR molecular marker,to analyze the extent of selfing depression in Liriodendron. The aim of this study is to explore the underlying mechanism of selfing depression. The results showed that the selfing depression in Liriodendron chinense was obvious. By taking progenies of intra-species cross and inter-species cross as references,the degree of depression (δ value) of selfing progenies for DBH,height and survival rate were 0. 46, 0. 45;0. 32,0. 35; and 0. 25,0. 30 respectively. SSR test results showed that the homozygous progeny rate derived from different mating types of selfing,intra-species,inter-species was from high to low trend. Moreover,we also detected 2 candidate SSR loci that might relate to recessive lethal gene in Liriodendron.