CAJ | 학술논문

【目的】明确来自广东和江西两省柑橘溃疡病菌菌株之间的分子水平差异．【方法】使用ERIC引物，通过REP-PCR技术，对来自我国广东和江西省12个市（县）12个柑橘品种的71个柑橘溃疡病菌株进行遗传多样性研究．根据DNA指纹特征，并用NTSYS软件聚类分析．【结果和结论】71个菌株在相似值为0.64时，可以分为A、B两簇，其中来自江西赣州6个市县的所有溃疡病菌株全部聚在A簇，而来自广东有50％的菌株聚在B簇．取相似值为0.88时，A、B簇又各自分为6个遗传组．广东和江西两省柑橘溃疡病菌存在明显的遗传多样性，不同地理来源和不同柑橘品种之间的溃疡病细菌多样性差异明显．
【목적】명학래자엄동화강서량성감귤궤양병균균주지간적분자수평차이．【방법】사용ERIC인물，통과REP-PCR기술，대래자아국엄동화강서성12개시（현）12개감귤품충적71개감귤궤양병균주진행유전다양성연구．근거DNA지문특정，병용NTSYS연건취류분석．【결과화결론】71개균주재상사치위0.64시，가이분위A、B량족，기중래자강서공주6개시현적소유궤양병균주전부취재A족，이래자엄동유50％적균주취재B족．취상사치위0.88시，A、B족우각자분위6개유전조．엄동화강서량성감귤궤양병균존재명현적유전다양성，불동지리래원화불동감귤품충지간적궤양병세균다양성차이명현．
Objective] Citrus canker caused by Xanthomonas citri subsp .citri ( Xcci) is one of the most important plant diseases .The aim of the experiment was to study the genetic diversities of Xac in Guang-dong and Jiangxi Provinces .[Method] The bacterial strains which were collected from 12 cities ( coun-ties) of Guangdong and Jiangxi Provinces on 12 varieties were investigated with ERIC-PCR.Dendrograms were generated by NTSYS software according to fingerprint features .[Result and conclusion] The results indicated that 71 Xac strains could be clustered division A and B .All the strains from Jiangxi Province were distributed on division A , while half of the strains from Guangdong Province were distributed on di-vision B .Both division A and B could be seperated into 6 groups at a level of 88%similarity respective-ly.There were significant genetic diversities of Xac strains from Guangdong and Jiangxi Provinces .It showed that the diversity differences of strains from different geographical origins or different varieties were significant .