CAJ | 학술논문

【目的】利用已完成测序的番茄基因组发展大量的SSR标记，并将这些标记转移到茄子及其他茄科作物上，节省开发SSR标记的成本．【方法】本研究利用近缘物种转移法分析了番茄SSR标记在茄子及其他茄科作物上的通用性情况．【结果和结论】1046对番茄SSR引物中有887对能在茄子基因组DNA上扩增出产物，425对引物扩增出的带型在番茄与茄子间相似程度高，标记的通用率为40．6％；EST-SSR比基因组SSR的通用性更好，前者通用率为54.5％，后者为38.9％；414个通用SSR标记被电子定位到番茄染色体上，不同染色体来源的标记通用率明显不同；93对引物在2份用于遗传图谱构建的栽培茄子亲本间表现出多态性；获得的425对通用引物在马铃薯、辣椒、枸杞上通用率分别为96.2％、78.1％、54.1％．
【목적】이용이완성측서적번가기인조발전대량적SSR표기，병장저사표기전이도가자급기타가과작물상，절성개발SSR표기적성본．【방법】본연구이용근연물충전이법분석료번가SSR표기재가자급기타가과작물상적통용성정황．【결과화결론】1046대번가SSR인물중유887대능재가자기인조DNA상확증출산물，425대인물확증출적대형재번가여가자간상사정도고，표기적통용솔위40．6％；EST-SSR비기인조SSR적통용성경호，전자통용솔위54.5％，후자위38.9％；414개통용SSR표기피전자정위도번가염색체상，불동염색체래원적표기통용솔명현불동；93대인물재2빈용우유전도보구건적재배가자친본간표현출다태성；획득적425대통용인물재마령서、랄초、구기상통용솔분별위96.2％、78.1％、54.1％．
Objective] Tomato genome was sequenced and a large number of SSR markers could be ob-tained .Cross-species amplification of tomato SSR markers will be an economic method to add up the number of robust SSR markers in eggplant and Solanaceous vegetables .[Method] This study tested a large set of tomato SSR markers on eggplant and other Solanaceous vegetables by close relative species transfer method .[Result and conclusion] 887 out of 1046 tested tomato SSR markers could successfully amplify on eggplants, but only 425 produced very similar bands on tomatoes and eggplants , giving a transfer rate of 40.6%.EST-SSR markers were found to be more transferable than genomic SSR markers (54.5 and 38.9%respectively ) .414 transferable SSR markers were mapped electronically onto tomato genome and their distribution on individual chromosomes highly varied .93 SSR markers showed polymor-phism on two eggplant parents used for genetic map construction .When testing 425 cross-species SSR markers on potatoes , peppers and lycium , the transferability rates were 96.2%, 78.1% and 54.1%, respectively .