CAJ | 학술논문

【目的】研究水稻Oryza sativa和再力花Thalia dealbata在单、间作模式下叶片光系统Ⅱ（ PSⅡ）对镉（ Cd）胁迫的响应以及2种植物的镉吸收积累特征．【方法】使用PAM-2500便携式荧光仪测定不同处理下植物叶片的叶绿素荧光参数．采用HNO3-HClO4湿法消解处理样品，并用石墨炉原子吸收法测定不同处理下植物组织Cd的含量．【结果和结论】随Cd浓度增加，水稻Fv/Fm、YI、qP 和rETR显著下降，qN 和NPQ显著升高，表明PSⅡ光合活性受到Cd胁迫抑制，接受光强过高的情况下叶片通过增加热耗散来消耗过剩的光能以减弱PSⅡ的光抑制．YNO显著升高，表明高浓度Cd处理对水稻的PSⅡ造成损害，导致光反应中心吸收的光量子以非调节性能量耗散的份额增加．而相同浓度处理下再力花的Fv/Fm、YI、qP、rETR降幅明显低于水稻，同时叶片YNPQ升高，但YNO稳定在较低水平，表明其调节性能量耗散机制发挥主要作用，PSⅡ光合活性受抑制程度相对较低．间作下水稻的根、茎、叶Cd含量低于单作．同一Cd浓度的间作模式下，再力花Cd积累总量显著高于水稻．以上结果表明，再力花对高浓度Cd胁迫有较强的耐受性，与水稻间作能降低水稻植株Cd含量．
【목적】연구수도Oryza sativa화재력화Thalia dealbata재단、간작모식하협편광계통Ⅱ（ PSⅡ）대력（ Cd）협박적향응이급2충식물적력흡수적루특정．【방법】사용PAM-2500편휴식형광의측정불동처리하식물협편적협록소형광삼수．채용HNO3-HClO4습법소해처리양품，병용석묵로원자흡수법측정불동처리하식물조직Cd적함량．【결과화결론】수Cd농도증가，수도Fv/Fm、YI、qP 화rETR현저하강，qN 화NPQ현저승고，표명PSⅡ광합활성수도Cd협박억제，접수광강과고적정황하협편통과증가열모산래소모과잉적광능이감약PSⅡ적광억제．YNO현저승고，표명고농도Cd처리대수도적PSⅡ조성손해，도치광반응중심흡수적광양자이비조절성능량모산적빈액증가．이상동농도처리하재력화적Fv/Fm、YI、qP、rETR강폭명현저우수도，동시협편YNPQ승고，단YNO은정재교저수평，표명기조절성능량모산궤제발휘주요작용，PSⅡ광합활성수억제정도상대교저．간작하수도적근、경、협Cd함량저우단작．동일Cd농도적간작모식하，재력화Cd적루총량현저고우수도．이상결과표명，재력화대고농도Cd협박유교강적내수성，여수도간작능강저수도식주Cd함량．
Objective] An experiment was conducted to ascertain the effects of Cd stress on the PS Ⅱchlorophyll fluorescence parameters in leaves of Thalia dealbata and rice in both the intercropping and mono-cropping systems as well as the uptake and accumulation characteristics of Cd by T.dealbata and rice.[Method] The chlorophyll fluorescence parameters in the leaves under different treatments were measured with PAM-2100 Chlorophyll Fluorometer .The contents of Cd in the samples from different treatments were determined using Graphite Furnace Atomic Absorption Spectrometry after wet ashing with HNO3-HClO4 .[Result and conclusion]The Fv/Fm , YI , qP and rETR of the rice decreased with the elevat-ed Cd content in the soil while the qN , NPQ and YNPQ of the rice showed an opposite trend , indicating that the PSⅡphotosynthetic activity was inhibited under Cd stress , while the proportion of the heat dissipation increased to alleviate the photoinhibition of PSⅡunder the condition of excessive light interception .The YNO of the rice increased in a high level of Cd content , which indicated that the higher level of Cd stress induced damage to PSⅡreaction centres , and the quantum yield of non-regulated energy dissipation in-creased to a higher level under conditions of excessive light energy .The decrease of Fv/Fm , YI , qP and rETR in T.dealbata were much lower than those in rice with the same level of Cd stress .With a higher level of Cd stress, the YNPQ)in T.dealbata increased significantly while the variation of YNO) in T.dealba-ta was not obvious , which suggested that the regulatory energy dissipation mechanism played a major role in the protection of photosynthetic apparatus in Cd stress .At the same level of Cd content in soil , the se-quence of Cd content was root >stem >leaf in the rice and stem >root and leaf in T.dealbata.The Cd contents in the root , stem and leaf of rice in the intercropping system were , respectively , lower than those in the monoculture system .The amount of Cd uptake by T.dealbata was significantly higher than that of rice with the same level of Cd treatment .All these results suggested that the photosystem II reac-tion center of T.dealbata had a higher tolerance to the Cd stress , and the Cd content of rice in intercrop-ping system could be reduced .