CAJ | 학술논문

【目的】筛选对无乳链球菌Streptococcus agalactiae具有良好体外抑菌效果的中药．【方法】通过乙醇回流、超声波方法提取20味中药的抗菌活性部位，采用试管二倍稀释法测定黄连Coptis chinensis等20味中药提取物对无乳链球菌的最低抑菌浓度（ Minimal inhibitory concentration ，MIC）和最低杀菌浓度（ Minimal bactericidal concentration ， MBC），并评价抗菌活性较强的几味中药的体外联合抑菌效果．【结果和结论】乌梅Prunus mume、黄连、黄芩Scutel-laria baicalensis、虎杖Polygonum cuspidatum和地榆Sanguisorba officinalis 5味中药提取物对无乳链球菌的MIC范围为7.80～31.25 mg/mL．蒲公英Taraxacum mongolicum、茵陈Artemisia scoparia、秦皮Fraxinus rhynchophylla、艾叶Arte-misia argyi、黄柏 Phellodendron chinense、鱼腥草 Houttuynia cordata、紫花地丁 Violae yedoensis 和白头翁 Pulsatilla chinensis 8味中药提取物的MIC范围为62.50～125.00 mg/mL．何首乌Po．multiflorum、苦参Sophora flavescens、穿心莲Andrographis paniculata、杜仲Eucommia ulmoides、连翘Forsythia suspensa、金银花Lonicera japonica和夏枯草Prunel-la vulgaris 7味中药提取物的MIC范围为250.00～500.00 mg/mL．联合抑菌试验结果表明，黄芩、虎杖、地榆和黄连的联合抑菌指数（FICI）≤1，虎杖、黄芩和地榆的FICI＞2．黄连、黄芩、虎杖和地榆对无乳链球菌均具有很强的体外抗菌活性，地榆、黄连为相加作用，黄连、黄芩和虎杖为协同作用，地榆与黄芩、虎杖为拮抗作用．
【목적】사선대무유련구균Streptococcus agalactiae구유량호체외억균효과적중약．【방법】통과을순회류、초성파방법제취20미중약적항균활성부위，채용시관이배희석법측정황련Coptis chinensis등20미중약제취물대무유련구균적최저억균농도（ Minimal inhibitory concentration ，MIC）화최저살균농도（ Minimal bactericidal concentration ， MBC），병평개항균활성교강적궤미중약적체외연합억균효과．【결과화결론】오매Prunus mume、황련、황금Scutel-laria baicalensis、호장Polygonum cuspidatum화지유Sanguisorba officinalis 5미중약제취물대무유련구균적MIC범위위7.80～31.25 mg/mL．포공영Taraxacum mongolicum、인진Artemisia scoparia、진피Fraxinus rhynchophylla、애협Arte-misia argyi、황백 Phellodendron chinense、어성초 Houttuynia cordata、자화지정 Violae yedoensis 화백두옹 Pulsatilla chinensis 8미중약제취물적MIC범위위62.50～125.00 mg/mL．하수오Po．multiflorum、고삼Sophora flavescens、천심련Andrographis paniculata、두중Eucommia ulmoides、련교Forsythia suspensa、금은화Lonicera japonica화하고초Prunel-la vulgaris 7미중약제취물적MIC범위위250.00～500.00 mg/mL．연합억균시험결과표명，황금、호장、지유화황련적연합억균지수（FICI）≤1，호장、황금화지유적FICI＞2．황련、황금、호장화지유대무유련구균균구유흔강적체외항균활성，지유、황련위상가작용，황련、황금화호장위협동작용，지유여황금、호장위길항작용．
Objective] Screening for the traditional Chinese medicines which have good antibacterial effects on Streptococcus agalactiae in vitro.[Method] Using ethanol reflux and ultrasonic method , ex-tracts of 20 different traditional Chinese medicines were obtained .Minimal inhibitory concentration ( MIC) and minimal bactericidal concentration ( MBC) of Coptis chinensis and other 19 extracts of tradi-tional Chinese medicines on St.agalactiae were detected by macrodilution broth method , and combined antibacterial effects in vitro of them were also observed .[Result and conclusion] The MICs of the ex-tracts obtained from Prunus mume, Coptis chinensis, Scutellaria baicalensis , Polygonum cuspidatum and Sanguisorba officinalis against St.agalactiae ranged from 7.80 to 31.25 mg/mL.The MICs of the ex-tracts obtained from Taraxacum mongolicum, Artemisia scoparia, Fraxinus rhynchophylla, Artemisia arg-yi, Phellodendron chinense, Houttuynia cordata, Violae yedoensis and Pulsatilla chinensis ranged from 62.50 to 125.00 mg/mL.The MICs of the extracts obtained from Po.multiflorum, Sophora flavescens, Andrographis paniculata, Eucommia ulmoides, Forsythia suspensa, Lonicera japonica and Prunella vul-garis ranged from 250.00 to 500.00 mg/mL.The results of joint bacteriostatic test showed that the frac-tional inhibitory concentration index ( FICI) of the extracts obtained from Sc.baicalensis, Po.cuspida-tum, Sa.officinalis and C.chinensis was less than or equal to 1.The FICI of Po.cuspidatum, Sc.ba-icalensis and Sa.officinalis was more than 2.C.chinensis, Sc.baicalensis, Po.cuspidatum and Sa.offi-cinalis showed a good antibacterial effect against St.agalactiae in vitro.The combination of Sa.officina-lis and C.chinensis was characterized by a additive effect , whereas the combination of C.chinensis, Sc. baicalensis and Po.cuspidatum was characterized by a synergism effect .The combination of Sa.officina-lis and Sc.baicalensis, Po.cuspidatum showed antagonism effect .