CAJ | 학술논문

【目的】为兽医临床合理用药，进行头孢喹肟对金黄色葡萄球菌的最低抑菌浓度（ MIC）和防突变浓度（ MPC）研究．【方法】采用标准琼脂二倍稀释法，测定头孢喹肟对金黄色葡萄球菌的MIC和MIC99（抑制99％接种细菌生长的最低抑菌浓度）；使用肉汤法富集1010 CFU/mL金黄色葡萄球菌ATCC 29213和37株金黄色葡萄球菌临床分离菌，采用标准琼脂平板稀释法测定头孢喹肟对金黄色葡萄球菌的MPC，并计算选择指数（SI）．【结果和结论】头孢喹肟对金黄色葡萄球菌ATCC 29213的MIC和MIC99分别是0.5和0.4μg/mL， MPC为1.6μg/mL， SI （ MPC/MIC99）为4．头孢喹肟对37株临床分离的金黄色葡萄球菌的MIC50、MIC90和MPC90分别是0.50、1.00和5.12μg/mL；SI （ MPC90/MIC90）为5.12．头孢喹肟对金黄色葡萄球菌具有较强的抗菌活性，SI也较小，通过调整给药方案可以减少耐药突变株的产生．
【목적】위수의림상합리용약，진행두포규우대금황색포도구균적최저억균농도（ MIC）화방돌변농도（ MPC）연구．【방법】채용표준경지이배희석법，측정두포규우대금황색포도구균적MIC화MIC99（억제99％접충세균생장적최저억균농도）；사용육탕법부집1010 CFU/mL금황색포도구균ATCC 29213화37주금황색포도구균림상분리균，채용표준경지평판희석법측정두포규우대금황색포도구균적MPC，병계산선택지수（SI）．【결과화결론】두포규우대금황색포도구균ATCC 29213적MIC화MIC99분별시0.5화0.4μg/mL， MPC위1.6μg/mL， SI （ MPC/MIC99）위4．두포규우대37주림상분리적금황색포도구균적MIC50、MIC90화MPC90분별시0.50、1.00화5.12μg/mL；SI （ MPC90/MIC90）위5.12．두포규우대금황색포도구균구유교강적항균활성，SI야교소，통과조정급약방안가이감소내약돌변주적산생．
Objective] The minimal inhibitory concentration ( MIC) and the mutant prevention concentra-tion ( MPC) of cefquinome against Staphylococcus aureus were measured for the rational use in veterinary medicine .[Method] The MIC and MIC for 99%of input cells ( MIC99 ) of cefquinome against S.aureus were determined by agar two-fold dilution method .The S.aureus strains were enriched in broth , and the bacterial concentrations were adjusted to 1010 colony forming units ( CFU) per milliliter.The MPC of cefquinome against S.aureus was determined by agar plates dilution method , and the selection index (SI) was calculated.[Result and conclusion] The MIC, MIC99, MPC and SI (MPC/MIC99) of cefqui-nome against S.aureus strain ATCC 29213 were 0.5, 0.4 , 1.6 μg/mL and 4, respectively.The MIC50 , MIC90 , MPC90 and SI ( MPC90/MIC90 ) of cefquinome against S.aureus clinical isolates were 0.50, 1.00, 5.12μg/mL and 5.12, respectively .The results show that cefquinome has a higher activity against S.aureus and a smaller SI .Resistant mutant strains can be reduced by adjusting the dosage regimen .