中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2014年
5期
341-345
,共5页
洪华章%周凯%傅平%黄琪%王俊%袁喜红%李坚
洪華章%週凱%傅平%黃琪%王俊%袁喜紅%李堅
홍화장%주개%부평%황기%왕준%원희홍%리견
胃肿瘤%脾酪氨酸激酶%人类Runt相关转录因子3%DNA甲基化%肿瘤复发%肿瘤转移%诊断%预后
胃腫瘤%脾酪氨痠激酶%人類Runt相關轉錄因子3%DNA甲基化%腫瘤複髮%腫瘤轉移%診斷%預後
위종류%비락안산격매%인류Runt상관전록인자3%DNA갑기화%종류복발%종류전이%진단%예후
Stomach neoplasms%Syk kinase%Runx3 protein%DNA methylation%Neoplasm recurrence%Neoplasm metastasis%Diagnosis%Prognosis
目的:探讨抑癌基因脾酪氨酸激酶(Syk)和人类Runt相关转录因子3(Runx3)启动子甲基化与胃癌术后复发转移的关系。方法采用甲基化特异性PCR检测70例胃癌组织和癌旁胃正常组织中Syk和Runx3基因启动子甲基化状态。结果在70例胃癌组织中,Syk和Runx3基因启动子甲基化发生率分别为45.7%(32/70)和55.7%(39/70);在癌旁胃正常组织中,Syk和Runx3基因启动子甲基化发生率分别为0(0/70)和7.1%(5/70)。胃癌组织中Syk和Runx3基因启动子甲基化发生率明显高于癌旁胃正常组织(均P<0.001)。 Syk、Runx3基因启动子甲基化与肿瘤的分化程度、浸润深度、淋巴结转移和病理分期有关(均P<0.05)。32例Syk基因启动子甲基化患者中,有21例患者术后发生复发转移,复发转移发生率为65.6%(21/32);38例Syk基因启动子非甲基化患者中,仅有7例患者术后发生复发转移,复发转移发生率为18.4%(7/38),差异有统计学意义(P<0.001)。39例Runx3基因启动子甲基化患者中,有24例患者术后发生复发转移,复发转移发生率为61.5%(24/39);31例Runx3基因启动子非甲基化患者中,仅有4例患者术后发生复发转移,复发转移发生率为12.9%(4/31),差异有统计学意义(P<0.001)。结论 Syk和Runx3基因启动子甲基化与胃癌术后复发转移密切相关。 Syk和Runx3基因启动子甲基化的联合检测有助于胃癌术后复发转移的早期诊断和预后评估。
目的:探討抑癌基因脾酪氨痠激酶(Syk)和人類Runt相關轉錄因子3(Runx3)啟動子甲基化與胃癌術後複髮轉移的關繫。方法採用甲基化特異性PCR檢測70例胃癌組織和癌徬胃正常組織中Syk和Runx3基因啟動子甲基化狀態。結果在70例胃癌組織中,Syk和Runx3基因啟動子甲基化髮生率分彆為45.7%(32/70)和55.7%(39/70);在癌徬胃正常組織中,Syk和Runx3基因啟動子甲基化髮生率分彆為0(0/70)和7.1%(5/70)。胃癌組織中Syk和Runx3基因啟動子甲基化髮生率明顯高于癌徬胃正常組織(均P<0.001)。 Syk、Runx3基因啟動子甲基化與腫瘤的分化程度、浸潤深度、淋巴結轉移和病理分期有關(均P<0.05)。32例Syk基因啟動子甲基化患者中,有21例患者術後髮生複髮轉移,複髮轉移髮生率為65.6%(21/32);38例Syk基因啟動子非甲基化患者中,僅有7例患者術後髮生複髮轉移,複髮轉移髮生率為18.4%(7/38),差異有統計學意義(P<0.001)。39例Runx3基因啟動子甲基化患者中,有24例患者術後髮生複髮轉移,複髮轉移髮生率為61.5%(24/39);31例Runx3基因啟動子非甲基化患者中,僅有4例患者術後髮生複髮轉移,複髮轉移髮生率為12.9%(4/31),差異有統計學意義(P<0.001)。結論 Syk和Runx3基因啟動子甲基化與胃癌術後複髮轉移密切相關。 Syk和Runx3基因啟動子甲基化的聯閤檢測有助于胃癌術後複髮轉移的早期診斷和預後評估。
목적:탐토억암기인비락안산격매(Syk)화인류Runt상관전록인자3(Runx3)계동자갑기화여위암술후복발전이적관계。방법채용갑기화특이성PCR검측70례위암조직화암방위정상조직중Syk화Runx3기인계동자갑기화상태。결과재70례위암조직중,Syk화Runx3기인계동자갑기화발생솔분별위45.7%(32/70)화55.7%(39/70);재암방위정상조직중,Syk화Runx3기인계동자갑기화발생솔분별위0(0/70)화7.1%(5/70)。위암조직중Syk화Runx3기인계동자갑기화발생솔명현고우암방위정상조직(균P<0.001)。 Syk、Runx3기인계동자갑기화여종류적분화정도、침윤심도、림파결전이화병리분기유관(균P<0.05)。32례Syk기인계동자갑기화환자중,유21례환자술후발생복발전이,복발전이발생솔위65.6%(21/32);38례Syk기인계동자비갑기화환자중,부유7례환자술후발생복발전이,복발전이발생솔위18.4%(7/38),차이유통계학의의(P<0.001)。39례Runx3기인계동자갑기화환자중,유24례환자술후발생복발전이,복발전이발생솔위61.5%(24/39);31례Runx3기인계동자비갑기화환자중,부유4례환자술후발생복발전이,복발전이발생솔위12.9%(4/31),차이유통계학의의(P<0.001)。결론 Syk화Runx3기인계동자갑기화여위암술후복발전이밀절상관。 Syk화Runx3기인계동자갑기화적연합검측유조우위암술후복발전이적조기진단화예후평고。
Objective To investigate the relationship between aberrant methylation of Syk and Runx3 genes and recurrence and metastasis after resection of gastric cancer . Methods Applying methylation-specific polymerase chain reaction technique , promoter methylation of Syk and Runx 3 genes in the tumor tissues and adjacent normal tissues of gastric cancer patients were detected to investigate the relationship between methylation status of the promoter region of Syk and Runx 3 genes and postoperative recurrence and metastasis . Results In the 70 cases of gastric cancer , the frequencies of promoter methylation of Syk and Runx3 genes were 45.7%(32/70) and 55.7% (39/70) in gastric cancer, and 0 (0/70) and 7.1%(5/70), respectively, in the adjacent normal tissues .The rates of promoter methylation of Syk and Runx3 genes in the gastric cancers were significantly higher than that in the adjacent normal tissues (P<0.001 for all).The promoter methylation of Syk and Runx3 genes was significantly correlated with the degree of tumor differentiation , depth of invasion , lymph node metastasis and pathological staging (P <0.05 for all).The frequency of postoperative recurrence and metastasis in 32 patients with Syk promoter methylation was 65.6% ( 21/32 ) and that in 38 cases with Syk promoter unmethylation was 18.4%(7/38), showing a significant difference between the two subgroups (χ2 =16.13, P<0.001).The rate of postoperative recurrence and metastasis in 39 patients with Runx3 promoter methylation was 61.5%(24/39) and that in 31 patients with Runx3 promoter unmethylation was 12.9% (4/31, P <0.001). Conclusions The methylation of Syk and Runx 3 promoters plays an important role in postoperative recurrence and metastasis of gastric cancer .Combined detection of promoter methylation of Syk and Runx 3 genes is helpful for early diagnosis and evaluation of prognosis of gastric cancer .