中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2014年
5期
329-334
,共6页
徐昊平%郭睿%金冶宁%李彪
徐昊平%郭睿%金冶寧%李彪
서호평%곽예%금야저%리표
肺肿瘤%放射疗法%Egr1基因启动子%人纤溶酶原Kringle5%杆状病毒%小鼠,裸%小动物正电子发射计算机体层摄影
肺腫瘤%放射療法%Egr1基因啟動子%人纖溶酶原Kringle5%桿狀病毒%小鼠,裸%小動物正電子髮射計算機體層攝影
폐종류%방사요법%Egr1기인계동자%인섬용매원Kringle5%간상병독%소서,라%소동물정전자발사계산궤체층섭영
Lung neoplasms%Radiotherapy%Egr1 promoter%Kringle5%Baculovirus%Mice,nude%Micro positron emission tomography-computed tomography
目的:探讨靶向基因放射治疗在裸鼠肺腺癌模型中的联合抗肿瘤作用,以及应用小动物正电子发射计算机体层摄影( Micro-PET-CT)动态评价疗效的可行性。方法建立肺腺癌A549裸鼠模型,并分为重组杆状病毒联合放疗组(即重组杆状病毒瘤内注射+放疗组、重组杆状病毒尾静脉内注射+放疗组、重组杆状病毒肌内注射+放疗组)、单纯放疗组和对照组,计算其肿瘤生长抑制率和肿瘤生长延缓时间。采用Micro-PET-CT动态评价18 F-FDG代谢变化,采用免疫组化法检测肿瘤组织中血管内皮生长因子( VEGF)、CD31和Ki-67的表达情况。结果重组杆状病毒联合放疗组的肿瘤生长延缓时间>12 d,肿瘤抑制率>45%,高于单纯放疗组(P<0.05)。免疫组化结果显示,重组杆状病毒联合放疗组的VEGF、CD31和Ki-67表达显著低于单纯放疗组和对照组( P<0.05)。 Micro-PET-CT显像显示,重组杆状病毒联合放疗组的肿瘤最大标准摄取值( SUVmax )较对照组和单纯放疗组明显降低(P<0.05),且重组杆状病毒瘤内注射+放疗组、重组杆状病毒尾静脉内注射+放疗组、重组杆状病毒肌内注射+放疗组和单纯放疗组裸鼠治疗后肿瘤体积与SUVmax正相关( r分别为0.976、0.954、0.929和0.871,P<0.05)。结论重组杆状病毒Bac Egr1-K5联合放疗增强了抑制肺腺癌生长的作用, Egr1启动子的射线可诱导性实现了治疗的靶向性及可控性。 Micro-PET-CT显像结果与治疗效果相关性好,可用于活体肿瘤的功能评价。
目的:探討靶嚮基因放射治療在裸鼠肺腺癌模型中的聯閤抗腫瘤作用,以及應用小動物正電子髮射計算機體層攝影( Micro-PET-CT)動態評價療效的可行性。方法建立肺腺癌A549裸鼠模型,併分為重組桿狀病毒聯閤放療組(即重組桿狀病毒瘤內註射+放療組、重組桿狀病毒尾靜脈內註射+放療組、重組桿狀病毒肌內註射+放療組)、單純放療組和對照組,計算其腫瘤生長抑製率和腫瘤生長延緩時間。採用Micro-PET-CT動態評價18 F-FDG代謝變化,採用免疫組化法檢測腫瘤組織中血管內皮生長因子( VEGF)、CD31和Ki-67的錶達情況。結果重組桿狀病毒聯閤放療組的腫瘤生長延緩時間>12 d,腫瘤抑製率>45%,高于單純放療組(P<0.05)。免疫組化結果顯示,重組桿狀病毒聯閤放療組的VEGF、CD31和Ki-67錶達顯著低于單純放療組和對照組( P<0.05)。 Micro-PET-CT顯像顯示,重組桿狀病毒聯閤放療組的腫瘤最大標準攝取值( SUVmax )較對照組和單純放療組明顯降低(P<0.05),且重組桿狀病毒瘤內註射+放療組、重組桿狀病毒尾靜脈內註射+放療組、重組桿狀病毒肌內註射+放療組和單純放療組裸鼠治療後腫瘤體積與SUVmax正相關( r分彆為0.976、0.954、0.929和0.871,P<0.05)。結論重組桿狀病毒Bac Egr1-K5聯閤放療增彊瞭抑製肺腺癌生長的作用, Egr1啟動子的射線可誘導性實現瞭治療的靶嚮性及可控性。 Micro-PET-CT顯像結果與治療效果相關性好,可用于活體腫瘤的功能評價。
목적:탐토파향기인방사치료재라서폐선암모형중적연합항종류작용,이급응용소동물정전자발사계산궤체층섭영( Micro-PET-CT)동태평개료효적가행성。방법건립폐선암A549라서모형,병분위중조간상병독연합방료조(즉중조간상병독류내주사+방료조、중조간상병독미정맥내주사+방료조、중조간상병독기내주사+방료조)、단순방료조화대조조,계산기종류생장억제솔화종류생장연완시간。채용Micro-PET-CT동태평개18 F-FDG대사변화,채용면역조화법검측종류조직중혈관내피생장인자( VEGF)、CD31화Ki-67적표체정황。결과중조간상병독연합방료조적종류생장연완시간>12 d,종류억제솔>45%,고우단순방료조(P<0.05)。면역조화결과현시,중조간상병독연합방료조적VEGF、CD31화Ki-67표체현저저우단순방료조화대조조( P<0.05)。 Micro-PET-CT현상현시,중조간상병독연합방료조적종류최대표준섭취치( SUVmax )교대조조화단순방료조명현강저(P<0.05),차중조간상병독류내주사+방료조、중조간상병독미정맥내주사+방료조、중조간상병독기내주사+방료조화단순방료조라서치료후종류체적여SUVmax정상관( r분별위0.976、0.954、0.929화0.871,P<0.05)。결론중조간상병독Bac Egr1-K5연합방료증강료억제폐선암생장적작용, Egr1계동자적사선가유도성실현료치료적파향성급가공성。 Micro-PET-CT현상결과여치료효과상관성호,가용우활체종류적공능평개。
Objective To explore the combined anti-tumor effect of radiation therapy and gene-targeted suppression of tumor neovasculature in lung adenocarcinoma in vivo , and to explore the feasibility of micro-PET/CT in dynamic evaluation of treatment effectiveness .Methods Thirty 5-6-week old male BALB/c nude mice were used in this study . The mouse models of xenotransplanted human lung adenocarcinoma were divided into 5 groups at random , six mice in each group:the control group , radiation treatment alone group and three groups of recombinant baculovirus plus radiation treatment ( intratumoral injection, tail vein injection, and intramuscular injection).The tumor volume was measured every 2 days. Growth delay time ( GD ) and growth inhibition rate was calculated .FDG metabolism was evaluated by micro-PET-CT before and after treatment .The expressions of VEGF , CD31 and Ki-67 were detected by immunohistochemistry ( IHC) .Results The tumor growth delay was >12 days, and the tumor inhibition rate was >45%in the recombinant baculovirus combined with radiotherapy groups , significantly higher than that of the radiotherapy alone group (P<0.05).Immunohistochemical analysis showed that the expressions of VEGF, CD31 and Ki-67 were significantly lower than that in other groups (P<0.05).The micro-PET-CT assessment showed that the FDG-metabolism in the recombinant baculovirus combined with radiotherapy groups was significantly reduced (P<0.05), and the SUVmax (FDG metabolism) of transplanted tumors after treatment was also markedly decreased in comparison with that of the control group .The tumor volume after treatment was significantly correlated with SUVmax in the recombinant baculovirus intratumoral injection+radiotherapy group (r=0.976), recombinant baculovirus intravenous injection +radiotherapy group (r=0.954 ), recombinant baculovirus intramuscular injection +radiotherapy group ( r =0.929 ), and radiotherapy alone group (r=0.871,P<0.05).Conclusions The recombinant baculovirus containing Egr1 promoter and K5 gene combined with radiotherapy enhances the suppressing effect on the growth of lung adenocarcinoma in the tumor-bearing nude mice .The inducibility of Egr 1 promoter by radiation allows the targeting and controllability of treatment .Micro-PET-CT results have a good correlation with the treatment effectiveness.Therefore, it can be used in real-time evaluation of tumor metabolic function in vivo .