世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
5期
1172-1177
,共6页
黄高%何光志%宋贵喜%刘霞%曹峰%王文佳%刘育辰%杨长福%蔡琨%徐邦祥%覃川%万金伙%罗贞恋%黄建梅
黃高%何光誌%宋貴喜%劉霞%曹峰%王文佳%劉育辰%楊長福%蔡琨%徐邦祥%覃川%萬金夥%囉貞戀%黃建梅
황고%하광지%송귀희%류하%조봉%왕문가%류육신%양장복%채곤%서방상%담천%만금화%라정련%황건매
染矽尘%苗药雾化剂%SOD%MDA%Fas基因
染矽塵%苗藥霧化劑%SOD%MDA%Fas基因
염석진%묘약무화제%SOD%MDA%Fas기인
Silica dust suspension%Miao nationality herbs spray%SOD%MDA%Fas gene
目的：通过制备苗药组方雾化剂，观察苗药组方雾化剂对染矽尘大鼠血清 SOD 和 MDA 及肺组织细胞凋亡Fas和Caspase-3基因表达的影响。方法：健康 SD大鼠分成5组，其中4组进行染矽尘造模，待模型成功后随机选其中3组分别给予糖皮质激素雾化、苗药组方雾化剂雾化、苗药组方雾化加中药灌胃治疗，治疗40天后，采用 WST-1法检测超氧化物歧化酶（SOD），TBA 法检测丙二醛（MDA），提取实验大鼠肺脏组织 RNA ，采用反转录合成 cDNA ，通过 RT-PCR 技术检测 Fas、Caspase-3基因表达。结果：与染矽尘组比较，苗药组方雾化组大鼠血清中 SOD 活力明显升高（P约0.05），苗药组方雾化组大鼠血清中 MDA 含量明显降低（P约0.05），苗药组方雾化组大鼠肺组织 Fas 和 Caspase-3基因表达明显降低（P约0.05）。结论：苗药组方雾化组能增强染矽尘大鼠血清SOD活力，减少染矽尘大鼠血清MDA含量，显著降低大鼠肺组织Fas和Caspase-3基因表达。
目的：通過製備苗藥組方霧化劑，觀察苗藥組方霧化劑對染矽塵大鼠血清 SOD 和 MDA 及肺組織細胞凋亡Fas和Caspase-3基因錶達的影響。方法：健康 SD大鼠分成5組，其中4組進行染矽塵造模，待模型成功後隨機選其中3組分彆給予糖皮質激素霧化、苗藥組方霧化劑霧化、苗藥組方霧化加中藥灌胃治療，治療40天後，採用 WST-1法檢測超氧化物歧化酶（SOD），TBA 法檢測丙二醛（MDA），提取實驗大鼠肺髒組織 RNA ，採用反轉錄閤成 cDNA ，通過 RT-PCR 技術檢測 Fas、Caspase-3基因錶達。結果：與染矽塵組比較，苗藥組方霧化組大鼠血清中 SOD 活力明顯升高（P約0.05），苗藥組方霧化組大鼠血清中 MDA 含量明顯降低（P約0.05），苗藥組方霧化組大鼠肺組織 Fas 和 Caspase-3基因錶達明顯降低（P約0.05）。結論：苗藥組方霧化組能增彊染矽塵大鼠血清SOD活力，減少染矽塵大鼠血清MDA含量，顯著降低大鼠肺組織Fas和Caspase-3基因錶達。
목적：통과제비묘약조방무화제，관찰묘약조방무화제대염석진대서혈청 SOD 화 MDA 급폐조직세포조망Fas화Caspase-3기인표체적영향。방법：건강 SD대서분성5조，기중4조진행염석진조모，대모형성공후수궤선기중3조분별급여당피질격소무화、묘약조방무화제무화、묘약조방무화가중약관위치료，치료40천후，채용 WST-1법검측초양화물기화매（SOD），TBA 법검측병이철（MDA），제취실험대서폐장조직 RNA ，채용반전록합성 cDNA ，통과 RT-PCR 기술검측 Fas、Caspase-3기인표체。결과：여염석진조비교，묘약조방무화조대서혈청중 SOD 활력명현승고（P약0.05），묘약조방무화조대서혈청중 MDA 함량명현강저（P약0.05），묘약조방무화조대서폐조직 Fas 화 Caspase-3기인표체명현강저（P약0.05）。결론：묘약조방무화조능증강염석진대서혈청SOD활력，감소염석진대서혈청MDA함량，현저강저대서폐조직Fas화Caspase-3기인표체。
This study was aimed to prepare the spraying agent of prescriptions of Miao nationality herb and investigate the effect of Miao nationality herbs spray for serum SOD, MDA, and expression of Fas and Caspase-3 mRNA in lung tissues of silica-treated rats. The healthy SD rats were divided into 5 groups. Silica dust suspension was used in the model establishment of 4 groups. After the model was successfully established, 3 groups were randomly selected and given glucocorticoids atomization inhalation, Miao nationality herbs spray, Miao nationality herbs spray combined with intragastric administration of herbal medicine, respectively. After 40-day treatment, water-solubletetrazolium salt (WST-1) was used in the detection of serum superoxide dismutase (SOD). Thiobarbituric acid (TBA) was used in the detection of malondialdehyde (MDA). The mRNA expression variance of the Fas and Caspase-3 were detected by RT-PCR. The results showed that compared with the silica dust suspension group, the SOD activity of serum in the Miao nationality herbs spray group was significantly increased (P< 0.05). MDA content and the mRNA of Fas and Caspase-3 were significantly lower in the Miao nationality herbs spray group (P< 0.05). It was concluded that Miao nationality herbs spray group was able to increase the SOD activity of serum, decrease MDA content, and obviously decrease the expression of Fas and Caspase-3 of lung tissues among silica dust suspension rats.
