世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
5期
1147-1152
,共6页
李冰冰%殷沈华%唐德才%臧文华
李冰冰%慇瀋華%唐德纔%臧文華
리빙빙%은침화%당덕재%장문화
补气活血药%血管内皮细胞%迁移%划痕法
補氣活血藥%血管內皮細胞%遷移%劃痕法
보기활혈약%혈관내피세포%천이%화흔법
Couplet medicines of reinforcing qi and activating blood%vascular endothelial cell%migration%scratch method
目的:观察补气活血类中药含药血清对体外培养的人脐静脉血管内皮细胞(HUVEC)迁移的影响,并初步筛选出对 HUVEC 迁移能力具有较明显的促进、抑制作用的药物。方法:建立 HUVEC的体外培养的细胞模型,将赤芍、川芎、丹皮、丹参、莪术、三棱、黄芪、人参按照1:2,1:1,2:13种配比两两配伍组合,组成13对补气活血药药对,采用血清药理学方法,制备含药血清,应用划痕法观察不同活血化瘀药和补气活血药药对的10%含药血清对 HUVEC(5×105/mL 密度)作用24 h 后迁移能力的影响。结果:与空白血清组相比较,丹参组、川芎组、赤芍组,丹参黄芪(1:2)组、丹参黄芪(1:1)组、川芎黄芪(1:2)组对血管内皮细胞的迁移能力具有明显促进作用(P约0.05或 P约0.01);三棱组、莪术组、丹皮组,莪术黄芪(2:1)组、三棱人参(2:1)组、莪术黄芪(1:1)组对血管内皮细胞的迁移能力具有明显抑制作用(P约0.05或 P约0.01)。结论:不同的活血化瘀药和补气活血药配伍组成对 HUVEC 的迁移能力具有不同的促进或抑制作用,这可能是这些药物在细胞层面上促进或抑制血管生成的作用机制之一。
目的:觀察補氣活血類中藥含藥血清對體外培養的人臍靜脈血管內皮細胞(HUVEC)遷移的影響,併初步篩選齣對 HUVEC 遷移能力具有較明顯的促進、抑製作用的藥物。方法:建立 HUVEC的體外培養的細胞模型,將赤芍、川芎、丹皮、丹參、莪術、三稜、黃芪、人參按照1:2,1:1,2:13種配比兩兩配伍組閤,組成13對補氣活血藥藥對,採用血清藥理學方法,製備含藥血清,應用劃痕法觀察不同活血化瘀藥和補氣活血藥藥對的10%含藥血清對 HUVEC(5×105/mL 密度)作用24 h 後遷移能力的影響。結果:與空白血清組相比較,丹參組、川芎組、赤芍組,丹參黃芪(1:2)組、丹參黃芪(1:1)組、川芎黃芪(1:2)組對血管內皮細胞的遷移能力具有明顯促進作用(P約0.05或 P約0.01);三稜組、莪術組、丹皮組,莪術黃芪(2:1)組、三稜人參(2:1)組、莪術黃芪(1:1)組對血管內皮細胞的遷移能力具有明顯抑製作用(P約0.05或 P約0.01)。結論:不同的活血化瘀藥和補氣活血藥配伍組成對 HUVEC 的遷移能力具有不同的促進或抑製作用,這可能是這些藥物在細胞層麵上促進或抑製血管生成的作用機製之一。
목적:관찰보기활혈류중약함약혈청대체외배양적인제정맥혈관내피세포(HUVEC)천이적영향,병초보사선출대 HUVEC 천이능력구유교명현적촉진、억제작용적약물。방법:건립 HUVEC적체외배양적세포모형,장적작、천궁、단피、단삼、아술、삼릉、황기、인삼안조1:2,1:1,2:13충배비량량배오조합,조성13대보기활혈약약대,채용혈청약이학방법,제비함약혈청,응용화흔법관찰불동활혈화어약화보기활혈약약대적10%함약혈청대 HUVEC(5×105/mL 밀도)작용24 h 후천이능력적영향。결과:여공백혈청조상비교,단삼조、천궁조、적작조,단삼황기(1:2)조、단삼황기(1:1)조、천궁황기(1:2)조대혈관내피세포적천이능력구유명현촉진작용(P약0.05혹 P약0.01);삼릉조、아술조、단피조,아술황기(2:1)조、삼릉인삼(2:1)조、아술황기(1:1)조대혈관내피세포적천이능력구유명현억제작용(P약0.05혹 P약0.01)。결론:불동적활혈화어약화보기활혈약배오조성대 HUVEC 적천이능력구유불동적촉진혹억제작용,저가능시저사약물재세포층면상촉진혹억제혈관생성적작용궤제지일。
This study was aimed to observe the influence on migration ability of human umbilical vein endothelial cell (HUVEC) in vitro by couplet medicines of reinforcing qi and activating blood, and initially screen medicines with relative obvious promoting or inhibiting effect on migration ability of HUVEC. The model of HUVEC cultured in vitro was established. The Paeonia, Ligusticum chuanxiong, Cortex moutan, Salvia, curcuma zedoaria, Sparganium, Astragalus, and ginseng were combined in pairs with the proportion of 1:2, 1:1, 2:1. There were 13 couplet medicines of reinforcing qi and activating blood. Serum pharmacological method was used to prepare medicated serum of the couplet medicines of reinforcing qi and activating blood. Scratch method was used to detect the effect of medicated serum of these couplet medicines of reinforcing qi and activating blood and activate blood herbs on the migration a-bility of HUVEC (with the density of 5í105/mL) after 24 hours. The results showed that compared with the blood serum of the blank group, the Cortex moutan group, Ligusticum chuanxiong group, Paeonia group, Salvia and Astra-galus (1:2) group, Salvia and Astragalus (1:1) group, Ligusticum chuanxiong and Astragalus (1:2) group had obvious promoting effect on the migration ability of VEC (P < 0.05 or P < 0.01). The Sparganium group, curcuma zedoaria group, Cortex moutan group, curcuma zedoaria and Astragalus (2:1) group, Sparganium and ginseng (2:1) group, Spar-ganium and Astragalus (1:1) group had obvious inhibiting effect on the migration ability of VEC (P< 0.05 or P<0.01). It was concluded that different compatibility of medicines of reinforcing qi and activating blood and activate blood herbs had different promoting or inhibiting effect on migration ability of HUVEC. It may be related to the mechanism of action of these drugs on promoting or inhibiting angiogenesis at the cellular level.