CAJ | 학술논문

目的：探讨补肾益髓生血法再障大鼠含药血清对大鼠骨髓造血祖细胞增殖分化及其机制的影响。方法：60Co-γ射线联合环磷酰胺建立再障大鼠模型，以正常对照组、模型组、康力龙组、益髓生血组、温肾生血组和滋肾生血组复方中药灌胃大鼠，制备其含药血清培养正常大鼠骨髓细胞，培养体系中含药血清比例为20豫，进行骨髓造血祖细胞集落形成单位（CFU）计数；收集集落细胞，RT-PCR检测红系GA原TA-1 mRNA及粒系PU.1 mRNA表达。结果：与正常对照组相比，模型组骨髓有核细胞显著减少（P<0.01），造血祖细胞红系集落形成单位（CFU-E）、红系爆式集落形成单位（BFU-E）和粒单核系造血祖细胞集落（CFU-GM）数目均明显降低（P<0.01），GATA-1、PU.1 mRNA表达显著降低（P<0.01）；与模型组相比，各个治疗组大鼠骨髓有核细胞升高，造血祖细胞CFU-E、BFU-E和CFU-GM数目显著增加（P<0.01）；滋肾生血组CFU-E、BFU-E明显优于益髓生血组（P约0.01）；滋肾生血组CFU-GM优于益髓生血组、温肾生血组。各治疗组GATA-1、PU.1 mRNA表达明显高于模型组（P<0.01）；滋肾生血组GATA-1 mRNA表达明显高于益髓生血组、温肾生血组（P<0.05）；滋肾生血组PU.1 mRNA表达高于益髓生血组、温肾生血组。结论：补肾益髓生血法可能通过增加GATA-1、PU.1 mRNA表达而促进骨髓造血祖细胞增殖分化，其中滋肾生血法优于益髓生血法和温肾生血法。
목적：탐토보신익수생혈법재장대서함약혈청대대서골수조혈조세포증식분화급기궤제적영향。방법：60Co-γ사선연합배린선알건립재장대서모형，이정상대조조、모형조、강력룡조、익수생혈조、온신생혈조화자신생혈조복방중약관위대서，제비기함약혈청배양정상대서골수세포，배양체계중함약혈청비례위20예，진행골수조혈조세포집락형성단위（CFU）계수；수집집락세포，RT-PCR검측홍계GA원TA-1 mRNA급립계PU.1 mRNA표체。결과：여정상대조조상비，모형조골수유핵세포현저감소（P<0.01），조혈조세포홍계집락형성단위（CFU-E）、홍계폭식집락형성단위（BFU-E）화립단핵계조혈조세포집락（CFU-GM）수목균명현강저（P<0.01），GATA-1、PU.1 mRNA표체현저강저（P<0.01）；여모형조상비，각개치료조대서골수유핵세포승고，조혈조세포CFU-E、BFU-E화CFU-GM수목현저증가（P<0.01）；자신생혈조CFU-E、BFU-E명현우우익수생혈조（P약0.01）；자신생혈조CFU-GM우우익수생혈조、온신생혈조。각치료조GATA-1、PU.1 mRNA표체명현고우모형조（P<0.01）；자신생혈조GATA-1 mRNA표체명현고우익수생혈조、온신생혈조（P<0.05）；자신생혈조PU.1 mRNA표체고우익수생혈조、온신생혈조。결론：보신익수생혈법가능통과증가GATA-1、PU.1 mRNA표체이촉진골수조혈조세포증식분화，기중자신생혈법우우익수생혈법화온신생혈법。
This study was aimed to investigate the effect of Bu-Shen Y i-Sui Sheng-Xue (BSYSSX) method on pro-liferation and differentiation mechanisms of hematopoietic progenitor cells. The rat models were established by 60Co-γrays and cyclophosphamide. Compound Chinese medicine was gavaged to rats of the normal control group, model group, stanozolol group, Yi-Sui Sheng-Xue (YSSX) group, Wen-Shen Sheng-Xue(WSSX) group and Zi-Shen Sheng-Xue (ZSSX) group. Then, serum of rat was prepared. Rat bone marrow cells were incubated with AA rats serum ac-counted for 20% and the number of hematopoietic progenitor cells colony-forming units (CFU) were counted. The level of GATA-1 and PU.1 mRNA in colony cells were detected with RT-PCR. The results showed that compared with the normal control group, the number of bone marrow cells, CFU-E, BFU-E, CFU-GM, as well as the expres-sion of GATA-1 and PU.1 mRNA in the model group decreased significantly (P< 0.01). Compared with the model group, the number of bone marrow cells, CFU-E, BFU-E, CFU-GM of each treatment group were significantly in-creased (P< 0.01). CFU-E and BFU-E of the ZSSX group were better than the YSSX group (P < 0.01). CFU-GM of the ZSSX group was better than the YSSX group and the WSSX group. The expression of GATA-1 and PU.1 mR-NA in each treatment group were significantly higher than the model group (P< 0.01). The expression of GATA-1 mRNA in the ZSSX group was better than the YSSZ group and WSSX group (P< 0.05). The expression of PU.1 mR-NA in the ZSSX group was higher than the YSSX group and WSSX group. It was concluded that BSYSSX method may increase the expression of GATA-1 and PU.1 mRNA in order to promote the proliferation and differentiation of bone marrow hematopoietic progenitor cells. The ZSSX method was better than the YSSX method and WSSX method.