世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
5期
1060-1064
,共5页
魏茜%刘铜华%时晓娟%李朋收%魏颖%徐暾海
魏茜%劉銅華%時曉娟%李朋收%魏穎%徐暾海
위천%류동화%시효연%리붕수%위영%서돈해
尖叶假龙胆%口山酮苷%含量测定%HPLC
尖葉假龍膽%口山酮苷%含量測定%HPLC
첨협가룡담%구산동감%함량측정%HPLC
Gentianella acuta%Xanthone glycosides%content determination%HPLC
目的：建立HPLC法同时测定尖叶假龙胆中的1，5-dihydroxy-3-methoxyxanthone 8-O-β-D-glucopyranoside （F1）、1-hydroxy-3,4-dimethoxyxanthon 7-O-β-D-glucopyranoside （F2）、1,8-dihydroxy-3,4-dimethoxyxanthone 5-O-β-D-glucopyranoside（F3）3个[口山]酮苷的含量。方法：Thermo syncronis C18（4.6 mm×250 mm，5μm）色谱柱，乙腈-水（24.5：75.5，V/V）等度洗脱，流速1.0 mL·min-1，检测波长为254 nm，柱温为35℃。结果：F1、F2、F3分别在14.06~281.28μg·mL-1（R2=0.9997）、0.56~11.16μg·mL-1（R2=0.9998）、0.46~9.20μg·mL-1（R2=0.9999）范围内线性关系良好；平均回收率（n=9）分别为99.70%（RSD=1.06%）、99.78%（RSD=1.21%）、100.28%（RSD=1.15%）。结论：该方法能快速、简便地测定尖叶假龙胆中3个主要的[口山]酮苷的含量，为该药材的质量控制研究提供了参考。
目的：建立HPLC法同時測定尖葉假龍膽中的1，5-dihydroxy-3-methoxyxanthone 8-O-β-D-glucopyranoside （F1）、1-hydroxy-3,4-dimethoxyxanthon 7-O-β-D-glucopyranoside （F2）、1,8-dihydroxy-3,4-dimethoxyxanthone 5-O-β-D-glucopyranoside（F3）3箇[口山]酮苷的含量。方法：Thermo syncronis C18（4.6 mm×250 mm，5μm）色譜柱，乙腈-水（24.5：75.5，V/V）等度洗脫，流速1.0 mL·min-1，檢測波長為254 nm，柱溫為35℃。結果：F1、F2、F3分彆在14.06~281.28μg·mL-1（R2=0.9997）、0.56~11.16μg·mL-1（R2=0.9998）、0.46~9.20μg·mL-1（R2=0.9999）範圍內線性關繫良好；平均迴收率（n=9）分彆為99.70%（RSD=1.06%）、99.78%（RSD=1.21%）、100.28%（RSD=1.15%）。結論：該方法能快速、簡便地測定尖葉假龍膽中3箇主要的[口山]酮苷的含量，為該藥材的質量控製研究提供瞭參攷。
목적：건립HPLC법동시측정첨협가룡담중적1，5-dihydroxy-3-methoxyxanthone 8-O-β-D-glucopyranoside （F1）、1-hydroxy-3,4-dimethoxyxanthon 7-O-β-D-glucopyranoside （F2）、1,8-dihydroxy-3,4-dimethoxyxanthone 5-O-β-D-glucopyranoside（F3）3개[구산]동감적함량。방법：Thermo syncronis C18（4.6 mm×250 mm，5μm）색보주，을정-수（24.5：75.5，V/V）등도세탈，류속1.0 mL·min-1，검측파장위254 nm，주온위35℃。결과：F1、F2、F3분별재14.06~281.28μg·mL-1（R2=0.9997）、0.56~11.16μg·mL-1（R2=0.9998）、0.46~9.20μg·mL-1（R2=0.9999）범위내선성관계량호；평균회수솔（n=9）분별위99.70%（RSD=1.06%）、99.78%（RSD=1.21%）、100.28%（RSD=1.15%）。결론：해방법능쾌속、간편지측정첨협가룡담중3개주요적[구산]동감적함량，위해약재적질량공제연구제공료삼고。
To develop a method for determining 3 Xanthone glycosides (1, 5-dihydroxy- 3-methoxyxanthone 8-O-β-D-glucopyranoside(F1), 1-hydroxy-3, 4-dimethoxyxanthon 7-O-β-D-glucopyranoside (F2), 1, 8-dihydroxy-3, 4-dimethoxyxanthone 5-O-β- D-glucopyranoside (F3) of Gentianella acuta by HPLC. The Thermo syncronis C18 (4.6 mmí250 mm, 5 μm) was used for simultaneous determination of 3 Xanthone glycosides in G. acuta. Isocratic elution with water and acetonitrile was 75.5:24.5. The flow rate was 1.0 mL·min-1 and the detection wavelength was set at 254 nm. The column temperature was 35℃. The linear concentration ranges of F1,F2,F3 were 14.06 ~ 281.28 μg·mL-1 (R2=0.999 7),0.56~11.16 μg·mL-1 (R2=0.999 8),0.46~9.20 μg·mL-1 (R2=0.999 9), respectively; The average recov-eries (n = 9) were 99.70% (RSD=1.06%),99.78% (RSD=1.21%),100.28% (RSD=1.15%), respectively. The method is simple, accurate and sensitive, and can be used for the quality control of G. acuta as a reference.
