世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2014年
5期
1005-1009
,共5页
吴金娟%张赓%姜淼%刘涛
吳金娟%張賡%薑淼%劉濤
오금연%장갱%강묘%류도
改良三甲%脑脊液%海马神经元%IL-1α%IL-1β%IL-6
改良三甲%腦脊液%海馬神經元%IL-1α%IL-1β%IL-6
개량삼갑%뇌척액%해마신경원%IL-1α%IL-1β%IL-6
Modified San-Jia-San decoction%cerebrospinal fluid%hippocampal neuron%IL-1α%IL-1β%IL-6
目的：探讨改良三甲散对β淀粉样蛋白（Aβ25-35）所致海马神经元细胞损伤时细胞炎性因子IL-1α、IL-1β和IL-6的影响。方法：将原代培养的神经元细胞分为空白组、模型组、石杉碱甲组和改良三甲散低、中、高剂量组。选择性培养7~10天后吸去培养液，分别加入空白培养液、正常脑脊液（生理盐水组）、石杉碱甲脑脊液和中药脑脊液低、中和高剂量，加培养液补至等量，37℃孵育2 h。然后除空白组之外的各组加入经老化处理的Aβ25-35（终浓度为5μmol·L-1），建立AD细胞模型。空白组加入等量的培养液，继续孵育24 h后收集细胞上清液。采用酶联免疫吸附测定法（ELISA）检测IL-1α、IL-1β和IL-6的含量。结果：改良三甲散能降低细胞上清液中IL-1α、IL-1β和IL-6的含量。结论：改良三甲散脑脊液能有效抑制细胞炎性因子IL-1α、IL-1β和IL-6的释放，具有一定的抗炎效应，从而保护海马神经元细胞。
目的：探討改良三甲散對β澱粉樣蛋白（Aβ25-35）所緻海馬神經元細胞損傷時細胞炎性因子IL-1α、IL-1β和IL-6的影響。方法：將原代培養的神經元細胞分為空白組、模型組、石杉堿甲組和改良三甲散低、中、高劑量組。選擇性培養7~10天後吸去培養液，分彆加入空白培養液、正常腦脊液（生理鹽水組）、石杉堿甲腦脊液和中藥腦脊液低、中和高劑量，加培養液補至等量，37℃孵育2 h。然後除空白組之外的各組加入經老化處理的Aβ25-35（終濃度為5μmol·L-1），建立AD細胞模型。空白組加入等量的培養液，繼續孵育24 h後收集細胞上清液。採用酶聯免疫吸附測定法（ELISA）檢測IL-1α、IL-1β和IL-6的含量。結果：改良三甲散能降低細胞上清液中IL-1α、IL-1β和IL-6的含量。結論：改良三甲散腦脊液能有效抑製細胞炎性因子IL-1α、IL-1β和IL-6的釋放，具有一定的抗炎效應，從而保護海馬神經元細胞。
목적：탐토개량삼갑산대β정분양단백（Aβ25-35）소치해마신경원세포손상시세포염성인자IL-1α、IL-1β화IL-6적영향。방법：장원대배양적신경원세포분위공백조、모형조、석삼감갑조화개량삼갑산저、중、고제량조。선택성배양7~10천후흡거배양액，분별가입공백배양액、정상뇌척액（생리염수조）、석삼감갑뇌척액화중약뇌척액저、중화고제량，가배양액보지등량，37℃부육2 h。연후제공백조지외적각조가입경노화처리적Aβ25-35（종농도위5μmol·L-1），건립AD세포모형。공백조가입등량적배양액，계속부육24 h후수집세포상청액。채용매련면역흡부측정법（ELISA）검측IL-1α、IL-1β화IL-6적함량。결과：개량삼갑산능강저세포상청액중IL-1α、IL-1β화IL-6적함량。결론：개량삼갑산뇌척액능유효억제세포염성인자IL-1α、IL-1β화IL-6적석방，구유일정적항염효응，종이보호해마신경원세포。
This study was aimed to investigate effect on the expression of cell inflammatory factor IL-1α, IL-1β and IL-6 of modified San-Jia-San (SJS) decoction on cultured hippocampal neurons injury induced by β-amyloid 25-35 protein (Aβ25-35). Primary cultured hippocampal neurons were divided into the normal group, model group, huperzine A group, low-dose SJS group, middle-dose SJS group, and high-dose SJS group. After selective culture for 7~10 days and the absorption of culture fluid, the blank culture fluid, normal cerebrospinal fluid (normal saline group), huperzine A cerebrospinal fluid, and low-, middle- and high-dose SJS cerebrospinal fluid were added, respectively. The culture fluid was added up to an equivalent medium. The incubation was 2 h under the temperature of 37℃. Ex-cept the normal group, Aβ25-35 dealt with aging (the final concentration was 5 μmol/L) was added to other groups to establish AD cell model. An equivalent amount of culture fluid was added to the normal group. After 24 h of incuba-tion, the cell culture supernatant was collected. Enzyme-linked immunosorbent assay (ELISA) was used in the content detection of IL-1α, IL-1β and IL-6. The results showed that modified SJS decoction can decrease the content of IL-1α, IL-1β and IL-6 in the cell culture supernatant. It was concluded that the modified SJS decoction can effectively inhibit the expression of cell inflammatory factor IL-1α, IL-1β and IL-6. It had certain anti-inflammatory effect to protect hippocampal neurons.
