CAJ | 학술논문

目的：探讨三妙丸中二妙丸配伍牛膝的合理性。方法：首次采用高尿酸血症致尿酸盐结晶沉积于关节的急性痛风关节炎大鼠模型，观察三妙及缺牛膝三妙丸对血尿酸值、炎症因子IL-1β和IL-6及关节病理组织的影响；HPLC-ESI-MS/MS 分析给药模型大鼠血清和关节中移行成分及其离子峰强度的相对变化。结果：三妙及缺牛膝三妙丸均有显著的抗急性痛风炎症及降血尿酸作用，而三妙丸抑制痛风大鼠足肿胀、保护关节组织的炎症损伤及抑制炎症因子IL-1β水平优于缺牛膝三妙丸；三妙及缺牛膝三妙丸给药血清均检测出5个移行成分，关节中1个移行成分，其中2个为黄柏生物碱代谢物、2个为苍术糖苷类成分代谢物，且三妙方血清及关节中移行成分离子强度均高于缺牛膝三妙方。结论：牛膝有促进二妙丸中黄柏生物碱及苍术倍半类成分在血液及关节中的分布，增强抗急性痛风关节炎药作用。
목적：탐토삼묘환중이묘환배오우슬적합이성。방법：수차채용고뇨산혈증치뇨산염결정침적우관절적급성통풍관절염대서모형，관찰삼묘급결우슬삼묘환대혈뇨산치、염증인자IL-1β화IL-6급관절병리조직적영향；HPLC-ESI-MS/MS 분석급약모형대서혈청화관절중이행성분급기리자봉강도적상대변화。결과：삼묘급결우슬삼묘환균유현저적항급성통풍염증급강혈뇨산작용，이삼묘환억제통풍대서족종창、보호관절조직적염증손상급억제염증인자IL-1β수평우우결우슬삼묘환；삼묘급결우슬삼묘환급약혈청균검측출5개이행성분，관절중1개이행성분，기중2개위황백생물감대사물、2개위창술당감류성분대사물，차삼묘방혈청급관절중이행성분리자강도균고우결우슬삼묘방。결론：우슬유촉진이묘환중황백생물감급창술배반류성분재혈액급관절중적분포，증강항급성통풍관절염약작용。
This article was aimed to discuss the reasonability of combined use of Achyranthes bidentata and Er-Miao-W an (EMW) in San-Miao-W an (SMW). Urate crystals caused by hyperuricemia deposited in the joint rat model was established for the first time. The serum uric acid level, inflammatory factors of IL-1β and IL-6, pathological tissues of joint of SMW and SMW lacked A . bidentata (EMW) were investigated by acute gouty arthritis rat model. HPLC-ESI-MS/MS method was used to analyze constituents absorbed in serum and joints which were obtained from the rat model after oral administration of SMW and EMW. Comparison was made on the intensity of ion peaks of constituents between SMW and EMW. The results showed that SMW and EMW had prominent effect in treatment of acute gouty arthritis and reducing serum uric acid level. SMW had better effect on the inhibition of swollen limbs and inflammatory factor IL-1β as well as the protection of inflammatory injury of joint tissues compared with EMW. Five constituents in the serum and one constituent in joints were detected from the rat models after oral administration of SMW and EMW. Two of them were metabolites of alkaloid constituents in Phellodendron Chinese and two of them were metabolites of glycoside constituents in A trac tylode s lanc e a. The intensity of ion peaks of SMW was higher than EMW. It was concluded that A . bidentata can promote the distribution of alkaloids components in Phellodendron Chinese and glycoside components in A . lancea in EMW in the blood and joints, which enhanced the anti-inflammatory effect of acute gouty arthritis.