CAJ | 학술논문

为获得较高的蓝莓花青苷，通过单因素试验分别考察酶类型、酶用量、固液比、超声时间、超声强化时间和pH值对花青苷提取量的影响；以花青苷的提取量为指标，采用正交试验对其提取工艺进行优化。结果表明：各因素对蓝莓花青苷的影响依次是酶用量＞固液比＞超声强化时间＞超声时间；其最佳工艺为1）纤维素酶100 U/mL、固液比1∶10（溶液 pH 为4．8）、酶解1 h，超声提取20 min，2）补充90％酸性pH1．0乙醇溶液使体系乙醇体积分数为60％、40℃下、超声强化提取30 min。在该条件下的最大提取量为9．63 mg/g，提取率高达95．06％。
위획득교고적람매화청감，통과단인소시험분별고찰매류형、매용량、고액비、초성시간、초성강화시간화pH치대화청감제취량적영향；이화청감적제취량위지표，채용정교시험대기제취공예진행우화。결과표명：각인소대람매화청감적영향의차시매용량＞고액비＞초성강화시간＞초성시간；기최가공예위1）섬유소매100 U/mL、고액비1∶10（용액 pH 위4．8）、매해1 h，초성제취20 min，2）보충90％산성pH1．0을순용액사체계을순체적분수위60％、40℃하、초성강화제취30 min。재해조건하적최대제취량위9．63 mg/g，제취솔고체95．06％。
In order to obtain higher content of blueberry anthocyanin, types of enzyme, enzyme dosage,solid-liquid ratio,ultrasonic time,ultrasonic-enhanced time and pH value were studied by single factor experiment test.The extraction process was optimized by orthogonal experiment with amount of cyanine nucleoside as index. The results showed the significance of each factor in turn was enzyme dosage> liquid ratio> ultrasonic-enhancement time> ultrasonic time;the optimal extraction technology of anthocyanin was cellulose 100U/mL,liquid ratio 1∶10 (pH 4.8),hydrolysis 1 h,extraction time 15 min,adding 90% of the acid (pH1.0)ethanol solution,the system was 60% ethanol concentration, temperature 40℃,ultrasonic assisted extraction time 30 min,the extraction amount of anthocyanin was 9 .6 3 mg/g,extraction rate was up to 9 5 .0 6%.