贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2014年
5期
113-116
,共4页
3％广枯灵水剂%辣椒白绢病原菌%抑菌活性
3％廣枯靈水劑%辣椒白絹病原菌%抑菌活性
3％엄고령수제%랄초백견병원균%억균활성
3％ Guangkuling aqueous solution%Sclerotiumrolfsii%antifungal activity
为了解3％广枯灵水剂对辣椒白绢病的抑制作用，对其进行了室内抑菌的初步研究。结果表明，3％广枯灵水剂能明显抑制辣椒白绢病菌菌丝的生长，EC50为15．3μg/mL；3％广枯灵100μg/mL处理的菌丝出现弯曲、不规则膨大、打结和局部膨肿现象；广枯灵浓度高于6．25μg/mL，菌丝干重明显下降，EC50为9．46μg/mL，能显著抑制孢子萌发；广枯灵浓度为25μg/mL时，孢子几乎不萌发；广枯灵处理细胞膜通透性的作用随处理时间的延长而增大。
為瞭解3％廣枯靈水劑對辣椒白絹病的抑製作用，對其進行瞭室內抑菌的初步研究。結果錶明，3％廣枯靈水劑能明顯抑製辣椒白絹病菌菌絲的生長，EC50為15．3μg/mL；3％廣枯靈100μg/mL處理的菌絲齣現彎麯、不規則膨大、打結和跼部膨腫現象；廣枯靈濃度高于6．25μg/mL，菌絲榦重明顯下降，EC50為9．46μg/mL，能顯著抑製孢子萌髮；廣枯靈濃度為25μg/mL時，孢子幾乎不萌髮；廣枯靈處理細胞膜通透性的作用隨處理時間的延長而增大。
위료해3％엄고령수제대랄초백견병적억제작용，대기진행료실내억균적초보연구。결과표명，3％엄고령수제능명현억제랄초백견병균균사적생장，EC50위15．3μg/mL；3％엄고령100μg/mL처리적균사출현만곡、불규칙팽대、타결화국부팽종현상；엄고령농도고우6．25μg/mL，균사간중명현하강，EC50위9．46μg/mL，능현저억제포자맹발；엄고령농도위25μg/mL시，포자궤호불맹발；엄고령처리세포막통투성적작용수처리시간적연장이증대。
To explore the inhibition activity of 3% Guangkuling aqueous solution on S.rolfsii,the indoor inhibition activity was preliminarily studied.Results:3% Guangkuling aqueous solution displayed highly strong antifungal activity on hyphal growth of S.rolfsii,with 50% effective concentration(EC50)of 15.3μg/mL.Meanwhile,the hyphal morphology of S.rolfsii treated with 100μg/mL Guangkuling was observed bulged irregularly,knotted and twisted.The dry weight of S.rolfsii decreased obviously when the concentration of Guangkuling was over 6.25μg/mL ,the EC50 was 9.46 g/mL.Furthermore,3%Guangkuling aqueous solution could inhibit the germination of S.rolfsii spores evidently.Guangkuling affected the permeability of cell membrane clearly. Along with the extension of treatment time, the osmotic effect of electrolyte from mycelial cells increased continually.
