贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2014年
5期
69-72,77
,共5页
曹利雅%李齐激%薛琰%叶辉%王道平%杨小生
曹利雅%李齊激%薛琰%葉輝%王道平%楊小生
조리아%리제격%설염%협휘%왕도평%양소생
枳椇子%脂肪酸%抗氧化活性
枳椇子%脂肪痠%抗氧化活性
지구자%지방산%항양화활성
Hovenia dulcis%fatty acid%antioxidant activity
为全面开发利用枳椇,采用气质(GC-MS)联用技术对枳椇子油的总脂肪酸、结合型脂肪酸和游离型脂肪酸组成及相对含量进行测定,运用 DPPH 和 Fenton法对枳椇子油的体外抗氧化活性进行检测与评价。结果表明:枳椇子油中检出总脂肪酸15种,主要成分为亚油酸和(Z)-9-十八烯酸(油酸),其含量分别为28.472%和26.292%。枳椇子油中结合型脂肪酸与游离型脂肪酸的比例为48.5∶1,其中以(Z)-9-十八烯酸(油酸)为主要成分的结合型脂肪酸6种,以亚油酸为主要成分的游离型脂肪酸15种。枳椇子油的体外抗氧化活性结果显示,对DPPH·自由基的清除能力随着浓度增加呈上升趋势,当浓度为15 mg/mL时,清除率为91.3%;对羟自由基清除能力呈明显量效关系,当浓度为6 mg/mL时,清除率为91.7%,均较橄榄油效果好。
為全麵開髮利用枳椇,採用氣質(GC-MS)聯用技術對枳椇子油的總脂肪痠、結閤型脂肪痠和遊離型脂肪痠組成及相對含量進行測定,運用 DPPH 和 Fenton法對枳椇子油的體外抗氧化活性進行檢測與評價。結果錶明:枳椇子油中檢齣總脂肪痠15種,主要成分為亞油痠和(Z)-9-十八烯痠(油痠),其含量分彆為28.472%和26.292%。枳椇子油中結閤型脂肪痠與遊離型脂肪痠的比例為48.5∶1,其中以(Z)-9-十八烯痠(油痠)為主要成分的結閤型脂肪痠6種,以亞油痠為主要成分的遊離型脂肪痠15種。枳椇子油的體外抗氧化活性結果顯示,對DPPH·自由基的清除能力隨著濃度增加呈上升趨勢,噹濃度為15 mg/mL時,清除率為91.3%;對羥自由基清除能力呈明顯量效關繫,噹濃度為6 mg/mL時,清除率為91.7%,均較橄欖油效果好。
위전면개발이용지구,채용기질(GC-MS)련용기술대지구자유적총지방산、결합형지방산화유리형지방산조성급상대함량진행측정,운용 DPPH 화 Fenton법대지구자유적체외항양화활성진행검측여평개。결과표명:지구자유중검출총지방산15충,주요성분위아유산화(Z)-9-십팔희산(유산),기함량분별위28.472%화26.292%。지구자유중결합형지방산여유리형지방산적비례위48.5∶1,기중이(Z)-9-십팔희산(유산)위주요성분적결합형지방산6충,이아유산위주요성분적유리형지방산15충。지구자유적체외항양화활성결과현시,대DPPH·자유기적청제능력수착농도증가정상승추세,당농도위15 mg/mL시,청제솔위91.3%;대간자유기청제능력정명현량효관계,당농도위6 mg/mL시,청제솔위91.7%,균교감람유효과호。
For the development and utilization of H.dulcis composition and content constituents of fatty acids,conjoint fatty acids and content of free fatty acids were identified by GC-MS.The antioxidant activities of the H.dulcis constituents of fatty acids in vitro were tested by the Fenton and DPPH free radical (DPPH ).The results showed that 15 fatty acids were identified in seed oil of H.dulcis,in which the main components were the unsaturated fatty acids with linoleic acid and (Z)-9-Octadecenoic acid (oleic acid).The weight rate of conjoint and free fatty acids was 48.5∶1,in which 15 fatty acids exist in free acids with linolenic acid as main constituent and 6 conj ugated type fatty acids with a maj or component oleic acid.The results of antioxidant acidities showed that seed oil had does-effect relationship at the scavenging activities of hydroxyl radical and DPPH radical invitro.When the concentration of sample was 6 mg/mL, the hydroxyl radical scavenging rate was 91.7%;the concentration of sample was 15 mg/mL,DPPH radical scavenging rate was 9 1 .3%,and the above results were better than those of olive oil.