贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2014年
5期
1-6
,共6页
水稻%半矮秆%突变体%贵州
水稻%半矮稈%突變體%貴州
수도%반왜간%돌변체%귀주
rice%semi-dwarf%mutant%Guizhou
为增加黑糯种质材料,以甲基磺酸乙酯(EMS)诱变处理的贵州地方稻种平塘黑糯得到的半矮秆突变体,并对突变体相关性状进行观察测定。结果表明:1)筛选的黑糯稻半矮秆突变体bgsd-2株高为65.20 cm、剑叶长22.24 cm、总穗粒数166粒、有效粒数147粒,与平塘黑糯差异极显著;千粒重25.76 g,与平塘黑糯差异显著;其余性状两者无显著性差异。2)突变体bgsd-2对外源 GA敏感,微管免疫荧光定位检测发现外源GA3能恢复突变体bgsd-2根尖倾斜排列的微管至平塘黑糯排列方式。3)突变体bgsd-2与9311、平塘黑糯杂交的F1代均表现为高秆,显示bgsd-2为隐性突变体。
為增加黑糯種質材料,以甲基磺痠乙酯(EMS)誘變處理的貴州地方稻種平塘黑糯得到的半矮稈突變體,併對突變體相關性狀進行觀察測定。結果錶明:1)篩選的黑糯稻半矮稈突變體bgsd-2株高為65.20 cm、劍葉長22.24 cm、總穗粒數166粒、有效粒數147粒,與平塘黑糯差異極顯著;韆粒重25.76 g,與平塘黑糯差異顯著;其餘性狀兩者無顯著性差異。2)突變體bgsd-2對外源 GA敏感,微管免疫熒光定位檢測髮現外源GA3能恢複突變體bgsd-2根尖傾斜排列的微管至平塘黑糯排列方式。3)突變體bgsd-2與9311、平塘黑糯雜交的F1代均錶現為高稈,顯示bgsd-2為隱性突變體。
위증가흑나충질재료,이갑기광산을지(EMS)유변처리적귀주지방도충평당흑나득도적반왜간돌변체,병대돌변체상관성상진행관찰측정。결과표명:1)사선적흑나도반왜간돌변체bgsd-2주고위65.20 cm、검협장22.24 cm、총수립수166립、유효립수147립,여평당흑나차이겁현저;천립중25.76 g,여평당흑나차이현저;기여성상량자무현저성차이。2)돌변체bgsd-2대외원 GA민감,미관면역형광정위검측발현외원GA3능회복돌변체bgsd-2근첨경사배렬적미관지평당흑나배렬방식。3)돌변체bgsd-2여9311、평당흑나잡교적F1대균표현위고간,현시bgsd-2위은성돌변체。
In order to obtain new rice dwarf mutants,a semi-dwarf mutant,bgsd-2 was identified from mutant progeny of the Guizhou local black glutinous cultivar rice by ethyl methanesulfonate (EMS ) treatment.In this study,the morphology and cytology of bgsd-2 were investigated and analyzed.Results:1)bgsd-2 mutant was a semi-dwarf grains,the plant height was 65.20 cm,the Flag leaf length was 22.24 cm,the seeds number per panicle was 166,the fertile seeds number per panicle was 147,their differences reached the very significant level with the Guizhou local black glutinous cultivar rice,while 1000-grain weight reached the significant level.There was no significant difference among the other characters.2 ) The bgsd-2 mutant was sensitive to response of exogenous GA3 . Immunolocalization of cortical microtubule (CMT ) assays found that the oblique CMTs abnormal organizations in bgsd-2 could be restored after exogenous application of GA3 .The two mutants were crossed with 9311 and wild-type,all F1 progeny showed a height phenotype.These showed that bgsd-2 mutant was the cryptic mutant.