CAJ | 학술논문

从峨眉山采集健康的一把伞南星（Arisaema erubescens（Wall） Schott ）植株，采用稀释涂布平板法从植物的组织液中分离内生细菌，采用牛津杯法筛选对大肠杆菌（Escherichia coli ）有拮抗作用的抗性菌株。研究结果表明，从一把伞南星中分离得到内生细菌23株，经过初筛和复筛共得到2株对大肠杆菌有较强抑菌活性的内生细菌：YC06、YC09，这两个菌株的发酵产物对大肠杆菌也有抑菌作用，抑菌圈直径均在13.0 mm以上。
종아미산채집건강적일파산남성（Arisaema erubescens（Wall） Schott ）식주，채용희석도포평판법종식물적조직액중분리내생세균，채용우진배법사선대대장간균（Escherichia coli ）유길항작용적항성균주。연구결과표명，종일파산남성중분리득도내생세균23주，경과초사화복사공득도2주대대장간균유교강억균활성적내생세균：YC06、YC09，저량개균주적발효산물대대장간균야유억균작용，억균권직경균재13.0 mm이상。
Endophytic bacteria were isolated from tissue of Healthy Arisaema erubescens (Wall) Schott plants collected from Mountain Emei by dilution spread plate method. Strains antagonistic against Escherichia coli were screened by Oxford Cup screening method. The results shows that 23 strains are isolated from A. erubescens, 2 strains (YC06, YC09) exhibits significant inhibitory activity against E. coli in primary and secondary screening. Ferment broth of 2 strains also has inhibitory activity against E. coli, inhibition zone diameter is 13.0 mm or more.