CAJ | 학술논문

目的：利用细胞内脱氢酶对2，3，5-三苯基氯化四氮唑（TTC）的催化作用建立比目前常用的3-（4，5-二甲基-2-噻唑）-2，5-二苯基溴化四氮唑（噻唑蓝，MTT）法更可靠的测定细胞活力的方法。方法使用不同浓度的TTC溶液测定细胞活力，并确定TTC的有效浓度范围。在计算所测定值与细胞数量函数关系的基础上，通过与传统测定细胞活力的MTT法进行比较，分析该方法的可行性。结果0．5％～1．0％浓度范围内的TTC溶液可用来测定细胞活力，测定值与细胞数量之间存在线性关系，与MTT法得到的结果基本一致，可避免MTT法测定过程中来自于细胞培养体系中其他抗氧化物质的非特异性干扰。结论 TTC可用于测定细胞活力，可替代常规的MTT法，具有操作简便，费用经济，结果可靠等优点。
목적：이용세포내탈경매대2，3，5-삼분기록화사담서（TTC）적최화작용건립비목전상용적3-（4，5-이갑기-2-새서）-2，5-이분기추화사담서（새서람，MTT）법경가고적측정세포활력적방법。방법사용불동농도적TTC용액측정세포활력，병학정TTC적유효농도범위。재계산소측정치여세포수량함수관계적기출상，통과여전통측정세포활력적MTT법진행비교，분석해방법적가행성。결과0．5％～1．0％농도범위내적TTC용액가용래측정세포활력，측정치여세포수량지간존재선성관계，여MTT법득도적결과기본일치，가피면MTT법측정과정중래자우세포배양체계중기타항양화물질적비특이성간우。결론 TTC가용우측정세포활력，가체대상규적MTT법，구유조작간편，비용경제，결과가고등우점。
Objective To develop a precise and reliable method to determine mammalian cell survival using the triphe-nyltetrazolium chloride (TTC) solution.Methods Different amounts of TTC solution were added to the cultured cells in order to find the optimal concentration .The correlation between the cell number and the measured optic density value was analyzed.In addition, the TTC method was compared with traditional MTT assay .Results The TTC assay could precisely reveal the viability of cultured cells as well as the MTT method , but the non-specific reaction of the MTT reagent by other antioxidants could result in unreliable overestimation of the cell number .Conclusion The TTC assay can be used to deter-mine the survival of cultured cells more precisely .