军事医学
軍事醫學
군사의학
BULLETIN OF THE ACADEMY OF MILITARY MEDICAL SCIENCES
2014年
5期
321-326
,共6页
张晶%张紫燕%杨传俊%蔡澎
張晶%張紫燕%楊傳俊%蔡澎
장정%장자연%양전준%채팽
极低频电磁场%高温%黑腹果蝇%hsp22%hsp26
極低頻電磁場%高溫%黑腹果蠅%hsp22%hsp26
겁저빈전자장%고온%흑복과승%hsp22%hsp26
extremely low frequency electromagnetic fields%high temperature%Drosophila melanogaster%hsp22%hsp26
目的:探讨在高温(35℃)条件下,极低频电磁场( extremely low frequency electromagnetic fields ,ELF-EMF)对雌雄黑腹果蝇hsp22和hsp26基因表达水平的影响。方法在25、27.5、30、32.5、35℃下,1日龄雌、雄黑腹果蝇在50 Hz、3 mT ELF-EMF暴露2 h,测定其死亡率、运动能力和hsp22、hsp26表达水平;在25和35℃下,1日龄雌、雄黑腹果蝇在50 Hz、3 mT ELF-EMF暴露2、4、8、24 h,测定其hsp22、hsp26表达水平。结果在25、27.5、30、32.5、35℃下暴露2 h,温度变化对果蝇的死亡率、运动能力和hsp22、hsp26表达水平有极显著影响(P<0.01),ELF-EMF对hsp22、hsp26表达水平无显著影响( P>0.05);在25和35℃下分别暴露2、4、8、24 h,性别、温度、ELF-EMF暴露和暴露时间对hsp22、hsp26的表达水平有极显著影响,其中,温度是主要的影响因素,且若干因素间有交互作用。结论 ELF-EMF可影响果蝇hsp22、hsp26的表达,且ELF-EMF可能具有降低因高温环境所致负面影响的作用,提高果蝇对高温环境的耐受能力,缓解高温环境对果蝇的损伤效应。
目的:探討在高溫(35℃)條件下,極低頻電磁場( extremely low frequency electromagnetic fields ,ELF-EMF)對雌雄黑腹果蠅hsp22和hsp26基因錶達水平的影響。方法在25、27.5、30、32.5、35℃下,1日齡雌、雄黑腹果蠅在50 Hz、3 mT ELF-EMF暴露2 h,測定其死亡率、運動能力和hsp22、hsp26錶達水平;在25和35℃下,1日齡雌、雄黑腹果蠅在50 Hz、3 mT ELF-EMF暴露2、4、8、24 h,測定其hsp22、hsp26錶達水平。結果在25、27.5、30、32.5、35℃下暴露2 h,溫度變化對果蠅的死亡率、運動能力和hsp22、hsp26錶達水平有極顯著影響(P<0.01),ELF-EMF對hsp22、hsp26錶達水平無顯著影響( P>0.05);在25和35℃下分彆暴露2、4、8、24 h,性彆、溫度、ELF-EMF暴露和暴露時間對hsp22、hsp26的錶達水平有極顯著影響,其中,溫度是主要的影響因素,且若榦因素間有交互作用。結論 ELF-EMF可影響果蠅hsp22、hsp26的錶達,且ELF-EMF可能具有降低因高溫環境所緻負麵影響的作用,提高果蠅對高溫環境的耐受能力,緩解高溫環境對果蠅的損傷效應。
목적:탐토재고온(35℃)조건하,겁저빈전자장( extremely low frequency electromagnetic fields ,ELF-EMF)대자웅흑복과승hsp22화hsp26기인표체수평적영향。방법재25、27.5、30、32.5、35℃하,1일령자、웅흑복과승재50 Hz、3 mT ELF-EMF폭로2 h,측정기사망솔、운동능력화hsp22、hsp26표체수평;재25화35℃하,1일령자、웅흑복과승재50 Hz、3 mT ELF-EMF폭로2、4、8、24 h,측정기hsp22、hsp26표체수평。결과재25、27.5、30、32.5、35℃하폭로2 h,온도변화대과승적사망솔、운동능력화hsp22、hsp26표체수평유겁현저영향(P<0.01),ELF-EMF대hsp22、hsp26표체수평무현저영향( P>0.05);재25화35℃하분별폭로2、4、8、24 h,성별、온도、ELF-EMF폭로화폭로시간대hsp22、hsp26적표체수평유겁현저영향,기중,온도시주요적영향인소,차약간인소간유교호작용。결론 ELF-EMF가영향과승hsp22、hsp26적표체,차ELF-EMF가능구유강저인고온배경소치부면영향적작용,제고과승대고온배경적내수능력,완해고온배경대과승적손상효응。
Objective To study the combined effect of extremely low frequency electromagnetic fields ( ELF-EMF ) exposure (50 Hz and 3 mT) and high temperature exposure on hsp22 and hsp26 expression in Drosophila melanogaster. Methods Under the conditions of 50 Hz and 3 mT ELF-EMF,the male and female D.melanogaster was separately exposed for 2 h at 25℃, 27.5℃, 30℃, 32.5℃,and 35℃, respectively.The death rate, exercise capacity and the hsp22 and hsp26 expression levels of D.melanogaster were detected after exposure;or at 25℃and 35℃at 2 h, 4 h, 8 h, and 24 h, respectively.The hsp22 and hsp26 expression levels of D.melanogaster were tested after exposure .Results After exposure for 2 h at 25℃, 27.5℃, 30℃, 32.5℃, and 35℃, temperature had a significant effect (P<0.01) on the death rate, exercise capacity and the hsp22 and hsp26 expression levels of D.melanogaster, but ELF-EMF had no significant effect (P>0.05).After exposure for 2 h at 25℃, 27.5℃, 30℃, 32.5℃, and 35℃, the hsp22 and hsp26 expression levels of D.melanogaster were significantly impacted(P<0.01) by gender, temperature, ELF-EMF and exposure time, and tem-perature was the most important factor .Gender, temperature, ELF-EMF and exposure time had some interaction with the results.Conclusion ELF-EMF can influence the expression levels of hsp22 and hsp26.ELF-EMF exposure contributes to heat tolerance in D.melanogaster through accelerated expression of hsp22 and hsp26.