CAJ | 학술논문

以13株乳酸菌为出发菌株，20 W的紫外灯，距离20 cm，照射120 s实现诱变。将诱变育种后的菌株在液体MRS培养基中培养6，24 h后分别测定pH值和可滴定酸量，筛选出高产酸菌株17-5；通过MRS-溴甲酚紫平板培养菌落产粘情况和胞外多糖产生量测定来筛选出高产胞外多糖菌株26-1。单独或混合接种两株菌株用于发酵泡菜，以未诱变处理菌株为对照组。发酵完成后对泡菜进行物性测定，测定泡菜汁液粘度和pH。得出结论：接种该条件诱变后的乳酸菌发酵能明显改善泡菜品质，提高产品质量。
이13주유산균위출발균주，20 W적자외등，거리20 cm，조사120 s실현유변。장유변육충후적균주재액체MRS배양기중배양6，24 h후분별측정pH치화가적정산량，사선출고산산균주17-5；통과MRS-추갑분자평판배양균락산점정황화포외다당산생량측정래사선출고산포외다당균주26-1。단독혹혼합접충량주균주용우발효포채，이미유변처리균주위대조조。발효완성후대포채진행물성측정，측정포채즙액점도화pH。득출결론：접충해조건유변후적유산균발효능명현개선포채품질，제고산품질량。
In this experiment,13 strains of lactic acid bacteria are exposed to 20 W UV lamp with the distance of 20 cm,irradiating for 120 s to implement mutagenesis.Then 13 experimental strains are cultured in liquid MRS medium for 6 h and 24 h respectively to detect the pH and titratable acidity amount and screen out the high yield of lactic acid bacteria strain 1 7-5 .Through the observation of producing sticky situations of MRS-bromocresol purple medium and the detection of production of LABEPS,then get the high-yield exopolysaccharide strain 2 6-1 .Eventually,alone or mix inoculate the purpose stains for fermentation of pickles,and starting strains are as the control group.After fer-mentation,the physical properties,viscosity and pH of pickle juice are detected.The results show that lactic acid bacteria fermentation can significantly improve the quality of pickles.