CAJ | 학술논문

目的：运用超高效液相色谱/四级杆飞行时间质谱( ultra performance liquid chromatography time-of-flight mass spectrometer)技术,研究口服不同剂量的冰片对丹参酮ⅡA大鼠体内药代动力学的影响。方法大鼠分别口服丹参酮IIA及丹参酮IIA加冰片混悬液,用UPLC-TOF-MS方法检测丹参酮IIA的血药浓度,用DAS软件拟合,计算相关药动学参数。结果检测丹参酮IIA在血浆中0.6~0.01 mg·L-1线性良好(r=0.999,n=7)。不同剂量的冰片与丹参酮ⅡA联合使用可明显增大丹参酮ⅡA的血药浓度( Cmax ),同时药时曲线下面积(AUC)明显增加,特别是15 mg·kg-1冰片对丹参酮ⅡA药代动力学产生明显影响。结论冰片与丹参酮ⅡA的配伍合用可以明显提高丹参酮ⅡA的吸收,增加丹参酮ⅡA的口服生物利用度,表明复方丹参中丹参和冰片配伍的科学性,显示二者相须相使的配伍关系。
목적：운용초고효액상색보/사급간비행시간질보( ultra performance liquid chromatography time-of-flight mass spectrometer)기술,연구구복불동제량적빙편대단삼동ⅡA대서체내약대동역학적영향。방법대서분별구복단삼동IIA급단삼동IIA가빙편혼현액,용UPLC-TOF-MS방법검측단삼동IIA적혈약농도,용DAS연건의합,계산상관약동학삼수。결과검측단삼동IIA재혈장중0.6~0.01 mg·L-1선성량호(r=0.999,n=7)。불동제량적빙편여단삼동ⅡA연합사용가명현증대단삼동ⅡA적혈약농도( Cmax ),동시약시곡선하면적(AUC)명현증가,특별시15 mg·kg-1빙편대단삼동ⅡA약대동역학산생명현영향。결론빙편여단삼동ⅡA적배오합용가이명현제고단삼동ⅡA적흡수,증가단삼동ⅡA적구복생물이용도,표명복방단삼중단삼화빙편배오적과학성,현시이자상수상사적배오관계。
Aim An ultra performance liquid chroma-tography coupled with time-of-fight mass spectrometry ( UPLC-TOF-MS) technique was used to evaluate the effect of borneol on the pharmacokinetics of tanshinone IIA in rats after oral administration of tanshinone IIA with different doses of borneol. Methods The rats were orally administered with tanshinone IIA and the mixed suspension of tanshinone IIA coupled with bor-neol. Respectively, the concentrations of tanshinoneIIA were determined by an UPLC-TOF-MS method, and the pharmacokinetic parameters were calculated by the software of DAS. Results The good linearity range of tanshinone IIA in plasma was 0 . 6~0 . 01 mg <br> ·L-1(r=0.999,n=7). The compatibility of differ-ent doses of borneol and tanshinone IIA could increase the blood concentrations of tanshinone IIA ( Cmax ) , in the area under the plasma concentration-time curve ( AUC) increased significantly, especially when 15 mg ·kg-1 of borneol was orally administered with tanshi-none IIA. Conclusions Borneol is able to obviously enhance the absorption and the relative bioavailability of tanshinone IIA. The described herb-drug interac-tions might prove the scientific rationality of the com-patible ratio of traditional Chinese medicine.