CAJ | 학술논문

目的：探讨磷酸酶 PTPMeg2对 NIH3T3/STAT3CA细胞模型的增殖及 STAT3转录活性的影响。方法构建STAT3异常突变的细胞模型 NIH3T3/STAT3CA,MTT法和裸鼠异体移植瘤实验分别用于观察 PTPMeg2对 NIH3T3/STAT3CA细胞体外和体内增殖的影响,免疫共沉淀实验用于PTPMeg2与STAT3CA相互作用的研究,双荧光素酶报告基因法用于转录活性的研究。结果 PTPMeg2对NIH3T3/STAT3CA细胞在体外和体内都具有增殖抑制作用,与对照组比较均具有明显的统计学意义。 PTPMeg2对 NIH3T3/STAT3CA细胞的转录活性具有抑制作用,而PTPMeg2的突变体( PTPMeg2C515S)和 ShPTPMeg2则无效。结论 PT-PMeg2对NIH3T3/STAT3CA细胞具有增殖抑制作用,其作用机制可能与抑制STAT3的转录活性有关。
목적：탐토린산매 PTPMeg2대 NIH3T3/STAT3CA세포모형적증식급 STAT3전록활성적영향。방법구건STAT3이상돌변적세포모형 NIH3T3/STAT3CA,MTT법화라서이체이식류실험분별용우관찰 PTPMeg2대 NIH3T3/STAT3CA세포체외화체내증식적영향,면역공침정실험용우PTPMeg2여STAT3CA상호작용적연구,쌍형광소매보고기인법용우전록활성적연구。결과 PTPMeg2대NIH3T3/STAT3CA세포재체외화체내도구유증식억제작용,여대조조비교균구유명현적통계학의의。 PTPMeg2대 NIH3T3/STAT3CA세포적전록활성구유억제작용,이PTPMeg2적돌변체( PTPMeg2C515S)화 ShPTPMeg2칙무효。결론 PT-PMeg2대NIH3T3/STAT3CA세포구유증식억제작용,기작용궤제가능여억제STAT3적전록활성유관。
Aim To investigate the NIH3T3/STAT3CA cell proliferation ability and the STAT3 transcriptional activity affected by PTPMeg2 . Methods MTT assay and xenograft nude mice model were used to investigate the NIH3 T3/STAT3 CA cell proliferation inhibited by PTPMeg2 in vitro and in vivo. Co-immunoprecipitation assay was used to measure the interaction between PT-PMeg2 and STAT3CA. STAT3 transcriptional activity was measured by dual-luciferase assay. Results The NIH3 T3/STAT3 CA cell proliferation ability was signifi-cantly inhibited by PTPMeg2 in vitro and in vivo com-pared with the control group ( P <0.05 ) . The tran-scriptional activity was increased by PTPMeg2 , but not the PTPMeg2 mutant (PTPMeg2C515S) and the ShPT-PMeg2 . Conclusion PTPMeg2 plays a role in inhibi-ting the proliferation ability of NIH3 T3/STAT3 CA cells through inhibiting the STAT3 transcriptional activity.