CAJ | 학술논문

目的：利用小干扰RNA技术沉默Bmi-1基因的表达,探讨其对人鼻咽癌CNE-1生物学行为的影响。方法设计并化学合成了针对Bmi-1的小干扰RNA,转染具有高转移性的CNE-1细胞,利用 qRT-PCR 和 Western blot 分别检测Bmi-1基因 mRNA 和蛋白的表达水平。体外通过 Transwell小室、Matrigel胶模型﹑流式细胞术,观察Bmi-1被沉默后对鼻咽癌细胞株CNE-1生物学行为的影响。结果 qRT-PCR和Western结果显示,Bmi-1-siRNA转染组Bmi-1基因的表达水平被有效抑制。与对照组相比, Bmi-1-siRNA转染组的细胞侵袭迁移力明显下降,细胞被阻滞在 S期。结论抑制Bmi-1基因的表达可有效降低CNE-1细胞侵袭迁移的能力。
목적：이용소간우RNA기술침묵Bmi-1기인적표체,탐토기대인비인암CNE-1생물학행위적영향。방법설계병화학합성료침대Bmi-1적소간우RNA,전염구유고전이성적CNE-1세포,이용 qRT-PCR 화 Western blot 분별검측Bmi-1기인 mRNA 화단백적표체수평。체외통과 Transwell소실、Matrigel효모형﹑류식세포술,관찰Bmi-1피침묵후대비인암세포주CNE-1생물학행위적영향。결과 qRT-PCR화Western결과현시,Bmi-1-siRNA전염조Bmi-1기인적표체수평피유효억제。여대조조상비, Bmi-1-siRNA전염조적세포침습천이력명현하강,세포피조체재 S기。결론억제Bmi-1기인적표체가유효강저CNE-1세포침습천이적능력。
Aim To investigate the effect of small inter-ference RNA-mediated silcencing of the Bmi-1 gene on cell invasion and metastasis in human nasopharyngeal carcinoma cell line CNE-1 . Methods Chemically syn-thesized siRNA targeting the Bmi-1 gene was transfect-ed into CNE-1 cells, which had high invasive and me-tastatic potential. The expression of Bmi-1 mRNA and protein were detected by quantative Real-time PCR and Western blot, respectively. The effects of Bmi-1 knockdown on CNE-1 cells migration and invasion were analysied by Transwell migration assay and Matrigel in-vasion assay. Results Transfected with Bmi-1 siRNA significantly down-regulated the expression of Bmi-1 mRNA and protein as compared with the control group. CNE-1 cells transfected with Bmi-1 siRNA had lower levels of invasion and migration capacity than cells in the control group. Conclusion SiRNA-media-ted silencing of the Bmi-1 gene could significantly in-hibit cell migration and invasion in human nasopharyn-geal carcinoma cell line CNE-1 .