CAJ | 학술논문

目的：探讨黄芩苷对人皮肤鳞状细胞癌A431细胞增殖及迁移能力的影响及其机制。方法50 mg · L-1黄芩苷孵育A431细胞,Western blot检测细胞中cofilin-1蛋白的表达水平；设计合成cofilin-1特异性siRNA并转染A431细胞,使用CCK8法检测细胞增殖活性,细胞划痕试验检测细胞的迁移能力。结果 Western blot结果显示,与对照组相比,细胞cofilin-1蛋白表达下降了49.3%；单独黄芩苷处理和cofi-lin-1-siRNA沉默均可抑制A431细胞生长与迁移,合并cofi-lin-1-siRNA则明显增强黄芩苷的作用效能。结论黄芩苷可有效降低肿瘤细胞的增殖活性和迁移能力,cofilin-1基因可能成为增强药物作用的调控靶点之一。
목적：탐토황금감대인피부린상세포암A431세포증식급천이능력적영향급기궤제。방법50 mg · L-1황금감부육A431세포,Western blot검측세포중cofilin-1단백적표체수평；설계합성cofilin-1특이성siRNA병전염A431세포,사용CCK8법검측세포증식활성,세포화흔시험검측세포적천이능력。결과 Western blot결과현시,여대조조상비,세포cofilin-1단백표체하강료49.3%；단독황금감처리화cofi-lin-1-siRNA침묵균가억제A431세포생장여천이,합병cofi-lin-1-siRNA칙명현증강황금감적작용효능。결론황금감가유효강저종류세포적증식활성화천이능력,cofilin-1기인가능성위증강약물작용적조공파점지일。
Aim To investigate the effect of baicalin on cell proliferation and cell migration in human skin SCC A431 cell line. Methods The A431 cells were incu- <br> bated with 50 mg·L-1 baicalin. The protein level of cofilin-1 was assayed by Western blot. Cofilin-1 specific siRNA fragment was designed , synthesized and trans- <br> fected into A431 cells. The proliferative activity and migration ability of cells were assessed by CCK8 assay and scratch wound healing assay separately. ResultsWestern blot results showed that baicalin treatment in-hibited the cofilin-1 protein expression to 49.3% com-pared with the control group. Single baicalin treatment and cofilin-1 silencing could drease the A431 cell growth and migration. And cofilin-1 silencing signifi- <br> cantly enhanced the efficacy of baicalin. Conclusions Baicalin could significantly inhibit the tumor cell's growth and migration in the A431 cell line. And cofi-lin-1 might become the potential target gene to enhance the effect of anticancer drugs.