CAJ | 학술논문

目的：研究不同浓度的新疆黑蜂胶乙醇提取物对 Balb/c 小鼠淋巴细胞的增殖作用。方法新疆黑蜂胶经乙醇提取混合物，做倍比稀释。Balb/c小鼠脾淋巴细胞分别加入500μg/mL及倍比稀释至15．6μg/mL的黑蜂胶共6个测定浓度，同时设脂多糖对照组、刀豆蛋白A对照组和空白对照组。采用CCK-8测定不同浓度黑蜂胶在体外刺激脾淋巴细胞后的OD450值。结果500μg/mL至62．5μg/mL的黑蜂胶刺激小鼠淋巴细胞增殖的活性显著高于空白对照组（P＜0．05），但低于ConA对照组（P＜0．05）。黑蜂胶的免疫激活作用与其药物浓度呈正相关（r＝0．794，P＜0．05）。结论新疆黑蜂胶可非特异性的促进淋巴细胞显著增殖。
목적：연구불동농도적신강흑봉효을순제취물대 Balb/c 소서림파세포적증식작용。방법신강흑봉효경을순제취혼합물，주배비희석。Balb/c소서비림파세포분별가입500μg/mL급배비희석지15．6μg/mL적흑봉효공6개측정농도，동시설지다당대조조、도두단백A대조조화공백대조조。채용CCK-8측정불동농도흑봉효재체외자격비림파세포후적OD450치。결과500μg/mL지62．5μg/mL적흑봉효자격소서림파세포증식적활성현저고우공백대조조（P＜0．05），단저우ConA대조조（P＜0．05）。흑봉효적면역격활작용여기약물농도정정상관（r＝0．794，P＜0．05）。결론신강흑봉효가비특이성적촉진림파세포현저증식。
Objective To study the different concentrations of Xinjiang black propolis extracted by ethanol stimulate the spleen lymphocyte proliferation of Balb /c mice in vitro.Method Xinjiang black propolis did times dilution from 500μg/mL to 1.95μg/mL in each well,add ConA,LPS and cell buffer fluid,all wells culture with Balb/c mice spleen lymphocytes for 48 hours,using CCK-8 method to detect OD 450 value.Result Compared with control groups,Xinjiang black propolis was shown a stronger activity of lymphocyte proliferation at the concentration from 500ug/mL to 62.5ug/mL than blank control;however,its activity was weaker than ConA control. And its immunological functions were shown a positive correlation with its concentrations .Conclusion Xinjiang black propolis can promote lymphocyte proliferation signif-icantly as a nonspecific stimulator .