现代肿瘤医学
現代腫瘤醫學
현대종류의학
JOURNAL OF MODERN ONCOLOGY
2014年
6期
1363-1365
,共3页
结肠癌%CD44%预后%侵袭%病理特征
結腸癌%CD44%預後%侵襲%病理特徵
결장암%CD44%예후%침습%병리특정
colorectal cancer%CD44%prognosis%invasion%pathological feature
目的：检测CD44基因mRNA在结肠癌组织中的表达。方法：收集25例明确诊断的结肠癌组织及其相应癌旁组织，提取总RNA并逆转录成cDNA。采用Real-time PCR方法检测CD44基因在结肠癌组织和癌旁组织中的 mRNA水平。结果：CD44基因在结肠癌组织中的含量明显高于癌旁组织（ P ＜0.05）。CD44基因与患者的性别（ P ＝0.804）、年龄（P＝0.592）、分化（P＝0.443）、淋巴侵袭（P＝0.525）、静脉侵袭（ P＝0.592）、肿瘤大小（ P＝0.592）和病理分期（ P＝0.824）等因素无关。Kaplan-meier生存曲线分析显示CD44基因与结肠癌患者预后具有显著相关性（P＝0.041）。结论：CD44基因mRNA在结肠癌组织中表达异常升高，提示CD44可能与结肠癌的发生、发展有密切关系。
目的：檢測CD44基因mRNA在結腸癌組織中的錶達。方法：收集25例明確診斷的結腸癌組織及其相應癌徬組織，提取總RNA併逆轉錄成cDNA。採用Real-time PCR方法檢測CD44基因在結腸癌組織和癌徬組織中的 mRNA水平。結果：CD44基因在結腸癌組織中的含量明顯高于癌徬組織（ P ＜0.05）。CD44基因與患者的性彆（ P ＝0.804）、年齡（P＝0.592）、分化（P＝0.443）、淋巴侵襲（P＝0.525）、靜脈侵襲（ P＝0.592）、腫瘤大小（ P＝0.592）和病理分期（ P＝0.824）等因素無關。Kaplan-meier生存麯線分析顯示CD44基因與結腸癌患者預後具有顯著相關性（P＝0.041）。結論：CD44基因mRNA在結腸癌組織中錶達異常升高，提示CD44可能與結腸癌的髮生、髮展有密切關繫。
목적：검측CD44기인mRNA재결장암조직중적표체。방법：수집25례명학진단적결장암조직급기상응암방조직，제취총RNA병역전록성cDNA。채용Real-time PCR방법검측CD44기인재결장암조직화암방조직중적 mRNA수평。결과：CD44기인재결장암조직중적함량명현고우암방조직（ P ＜0.05）。CD44기인여환자적성별（ P ＝0.804）、년령（P＝0.592）、분화（P＝0.443）、림파침습（P＝0.525）、정맥침습（ P＝0.592）、종류대소（ P＝0.592）화병리분기（ P＝0.824）등인소무관。Kaplan-meier생존곡선분석현시CD44기인여결장암환자예후구유현저상관성（P＝0.041）。결론：CD44기인mRNA재결장암조직중표체이상승고，제시CD44가능여결장암적발생、발전유밀절관계。
Objective:To determine the expression of CD44 gene mRNA in colorectal cancer. Methods:Twenty five colorectal cancer tissues and matched normal tissues were collected by surgery,total RNA extraction and reverse transcription into cDNA. The levels of CD44 mRNA were detected by using Real-time PCR. Results:The levels of CD44 mRNA were higher in cancer tissues than that in normal tissues(P<0. 05). High CD44 expression was not as-sociated with sex(P=0. 804),age(P=0. 592),differentiation(P=0. 443),lymphatic invasion(P=0. 525),venous invasion(P=0. 592),tumor size(P=0. 592)and pN category(P=0. 824). CD44 expression was associated with sur-vival rate of the patients with colorectal cancer by using Kaplan-meier survival curve analysis(P=0. 041). Conclu-sion:Abnormal high CD44 mRNA expression was observed in colorectal cancer tissues,which suggest that there was association between CD44 and the development of colorectal cancer.
